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2ra5

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[[Image:2ra5.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor==
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|PDB= 2ra5 |SIZE=350|CAPTION= <scene name='initialview01'>2ra5</scene>, resolution 2.40&Aring;
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<StructureSection load='2ra5' size='340' side='right'caption='[[2ra5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene> and <scene name='pdbligand=IPA:ISOPROPYL ALCOHOL'>IPA</scene>
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<table><tr><td colspan='2'>[[2ra5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RA5 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= SCO6256 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ra5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ra5 OCA], [https://pdbe.org/2ra5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ra5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ra5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ra5 ProSAT], [https://www.topsan.org/Proteins/MCSG/2ra5 TOPSAN]</span></td></tr>
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</table>
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'''Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RKT6_STRCO Q9RKT6_STRCO]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2ra5_consurf.spt"</scriptWhenChecked>
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2RA5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RA5 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17982461 17982461]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ra5 ConSurf].
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[[Category: Bacteria]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Edwards, A M.]]
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</StructureSection>
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[[Category: Joachimiak, A.]]
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[[Category: Large Structures]]
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[[Category: Kim, Y.]]
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[[Category: Edwards AM]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Joachimiak A]]
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[[Category: Savchenko, A.]]
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[[Category: Kim Y]]
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[[Category: Xu, X.]]
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[[Category: Savchenko A]]
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[[Category: Zheng, H.]]
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[[Category: Xu X]]
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[[Category: IPA]]
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[[Category: Zheng H]]
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[[Category: SRT]]
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[[Category: beta structure]]
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[[Category: dna-binding]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: protein structure initiative]]
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[[Category: psi-2]]
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[[Category: structural genomic]]
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[[Category: transcription]]
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[[Category: transcription regulation]]
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[[Category: utra domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:33:59 2008''
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Current revision

Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor

PDB ID 2ra5

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