1gsj

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[[Image:1gsj.gif|left|200px]]<br />
 
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<applet load="1gsj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gsj, resolution 1.85&Aring;" />
 
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'''SELENOMETHIONINE SUBSTITUTED N-ACETYL-L-GLUTAMATE KINASE FROM ESCHERICHIA COLI COMPLEXED WITH ITS SUBSTRATE N-ACETYL-L-GLUTAMATE AND ITS SUBSTRATE ANALOG AMPPNP'''<br />
 
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==Overview==
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==Selenomethionine substituted N-acetyl-L-glutamate kinase from Escherichia coli complexed with its substrate n-acetyl-L-glutamate and its substrate analog AMPPNP==
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N-Acetyl-L-glutamate kinase (NAGK), a member of the amino acid kinase, family, catalyzes the second and frequently controlling step of arginine, synthesis. The Escherichia coli NAGK crystal structure to 1.5 A resolution, reveals a 258-residue subunit homodimer nucleated by a central 16-stranded, molecular open beta sheet sandwiched between alpha helices. In each, subunit, AMPPNP, as an alphabetagamma-phosphate-Mg2+ complex, binds along, the sheet C edge, and N-acetyl-L-glutamate binds near the dyadic axis with, its gamma-COO- aligned at short distance from the gamma-phosphoryl, indicating associative phosphoryl transfer assisted by: (1) Mg2+, complexation; (2) the positive charges on Lys8, Lys217, and on two helix, dipoles; and (3) by hydrogen bonding with the y-phosphate. The structural, resemblance with carbamate kinase and the alignment of the sequences, suggest that NAGK is a structural and functional prototype for the amino, acid kinase family, which differs from other acylphosphate-making devices, represented by phosphoglycerate kinase, acetate kinase, and biotin, carboxylase.
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<StructureSection load='1gsj' size='340' side='right'caption='[[1gsj]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GSJ FirstGlance]. <br>
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1GSJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, NLG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acetylglutamate_kinase Acetylglutamate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.8 2.7.2.8] Structure known Active Site: NLG. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GSJ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NLG:N-ACETYL-L-GLUTAMATE'>NLG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsj OCA], [https://pdbe.org/1gsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gsj RCSB], [https://www.ebi.ac.uk/pdbsum/1gsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsj ProSAT]</span></td></tr>
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Structure of acetylglutamate kinase, a key enzyme for arginine biosynthesis and a prototype for the amino acid kinase enzyme family, during catalysis., Ramon-Maiques S, Marina A, Gil-Ortiz F, Fita I, Rubio V, Structure. 2002 Mar;10(3):329-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12005432 12005432]
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</table>
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[[Category: Acetylglutamate kinase]]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/ARGB_ECOLI ARGB_ECOLI]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Fita, I.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Gil-Ortiz, F.]]
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Check<jmol>
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[[Category: Marina, A.]]
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<jmolCheckbox>
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[[Category: Ramon-Maiques, S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gsj_consurf.spt"</scriptWhenChecked>
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[[Category: Rubio, V.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: ANP]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: MG]]
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</jmolCheckbox>
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[[Category: NLG]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gsj ConSurf].
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[[Category: acetylglutamate kinase]]
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<div style="clear:both"></div>
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[[Category: amino acid kinase]]
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__TOC__
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[[Category: arginine biosynthesis]]
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</StructureSection>
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[[Category: carbamate kinase]]
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[[Category: Large Structures]]
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[[Category: phosphoryl group transfer]]
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[[Category: Fita I]]
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[[Category: protein crystallography]]
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[[Category: Gil-Ortiz F]]
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[[Category: selenomethionine]]
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[[Category: Marina A]]
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[[Category: Ramon-Maiques S]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:16:46 2007''
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[[Category: Rubio V]]

Current revision

Selenomethionine substituted N-acetyl-L-glutamate kinase from Escherichia coli complexed with its substrate n-acetyl-L-glutamate and its substrate analog AMPPNP

PDB ID 1gsj

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