4zb1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4zb1 is ON HOLD Authors: Lee, C.C., Ching, C.Y., Tsai, H.J., Wang, A.H.J. Description: Crystal Structure of Blue Chromoprotein sgBP from Stichodact...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Blue Chromoprotein sgBP from Stichodactyla Gigantea== | |
| + | <StructureSection load='4zb1' size='340' side='right'caption='[[4zb1]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4zb1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stichodactyla_gigantea Stichodactyla gigantea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZB1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=TOE:2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL'>TOE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zb1 OCA], [https://pdbe.org/4zb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zb1 RCSB], [https://www.ebi.ac.uk/pdbsum/4zb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zb1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0M3KL34_STIGI A0A0M3KL34_STIGI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chromoproteins (CPs) have unique colors and can be used in biological applications. In this work, a novel blue CP with a maximum absorption peak (lambdamax) at 608 nm was identified from the carpet anemone Stichodactyla gigantea (sgBP). In vivo expression of sgBP in zebrafish would change the appearance of the fishes to have a blue color, indicating the potential biomarker function. To enhance the color properties, the crystal structure of sgBP at 2.25 A resolution was determined to allow structure-based protein engineering. Among the mutations conducted in the Gln-Tyr-Gly chromophore and chromophore environment, a S157C mutation shifted the lambdamax to 604 nm with an extinction coefficient (epsilon) of 58,029 M-1.cm-1 and darkened the blue color expression. The S157C mutation in the sgBP chromophore environment could affect the color expression by altering the deprotonation state of the phenolic group in the chromophore. Our results provide a structural basis for the blue color enhancement of the biomarker development. | ||
| - | + | Chromophore Deprotonation State Alters the Optical Properties of Blue Chromoprotein.,Chiang CY, Lee CC, Lo SY, Wang AH, Tsai HJ PLoS One. 2015 Jul 28;10(7):e0134108. doi: 10.1371/journal.pone.0134108., eCollection 2015. PMID:26218063<ref>PMID:26218063</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Ching | + | <div class="pdbe-citations 4zb1" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Tsai | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Stichodactyla gigantea]] | ||
| + | [[Category: Ching CY]] | ||
| + | [[Category: Lee CC]] | ||
| + | [[Category: Tsai HJ]] | ||
| + | [[Category: Wang AHJ]] | ||
Current revision
Crystal Structure of Blue Chromoprotein sgBP from Stichodactyla Gigantea
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