2n1t
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Dynamic binding mode of a synaptotagmin-1-SNARE complex in solution== | |
| + | <StructureSection load='2n1t' size='340' side='right'caption='[[2n1t]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2n1t]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N1T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n1t OCA], [https://pdbe.org/2n1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n1t RCSB], [https://www.ebi.ac.uk/pdbsum/2n1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n1t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rapid neurotransmitter release depends on the Ca2+ sensor Synaptotagmin-1 (Syt1) and the SNARE complex formed by synaptobrevin, syntaxin-1 and SNAP-25. How Syt1 triggers release has been unclear, partly because elucidating high-resolution structures of Syt1-SNARE complexes has been challenging. An NMR approach based on lanthanide-induced pseudocontact shifts now reveals a dynamic binding mode in which basic residues in the concave side of the Syt1 C2B-domain beta-sandwich interact with a polyacidic region of the SNARE complex formed by syntaxin-1 and SNAP-25. The physiological relevance of this dynamic structural model is supported by mutations in basic residues of Syt1 that markedly impair SNARE-complex binding in vitro and Syt1 function in neurons. Mutations with milder effects on binding have correspondingly milder effects on Syt1 function. Our results support a model whereby dynamic interaction facilitates cooperation between Syt1 and the SNAREs in inducing membrane fusion. | ||
| - | + | Dynamic binding mode of a Synaptotagmin-1-SNARE complex in solution.,Brewer KD, Bacaj T, Cavalli A, Camilloni C, Swarbrick JD, Liu J, Zhou A, Zhou P, Barlow N, Xu J, Seven AB, Prinslow EA, Voleti R, Haussinger D, Bonvin AM, Tomchick DR, Vendruscolo M, Graham B, Sudhof TC, Rizo J Nat Struct Mol Biol. 2015 Jun 1. doi: 10.1038/nsmb.3035. PMID:26030874<ref>PMID:26030874</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[ | + | </div> |
| - | [[ | + | <div class="pdbe-citations 2n1t" style="background-color:#fffaf0;"></div> |
| - | [[ | + | |
| - | [[ | + | ==See Also== |
| - | [[Category: | + | *[[Synaptosomal-associated protein|Synaptosomal-associated protein]] |
| - | [[Category: | + | *[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]] |
| - | [[Category: | + | *[[Syntaxin 3D structures|Syntaxin 3D structures]] |
| - | [[Category: Bacaj | + | *[[Vesicle-associated membrane protein 3D structures|Vesicle-associated membrane protein 3D structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Brewer | + | __TOC__ |
| - | [[Category: Camilloni | + | </StructureSection> |
| - | [[Category: Cavalli | + | [[Category: Homo sapiens]] |
| - | [[Category: Graham | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Rattus norvegicus]] |
| - | [[Category: | + | [[Category: Bacaj T]] |
| - | [[Category: | + | [[Category: Barlow N]] |
| - | [[Category: Sudhof | + | [[Category: Bonvin A]] |
| - | [[Category: | + | [[Category: Brewer K]] |
| - | [[Category: | + | [[Category: Camilloni C]] |
| - | [[Category: | + | [[Category: Cavalli A]] |
| + | [[Category: Graham B]] | ||
| + | [[Category: Haussinger D]] | ||
| + | [[Category: Liu J]] | ||
| + | [[Category: Prinslow E]] | ||
| + | [[Category: Rizo J]] | ||
| + | [[Category: Seven A]] | ||
| + | [[Category: Sudhof T]] | ||
| + | [[Category: Swarbrick J]] | ||
| + | [[Category: Tomchick D]] | ||
| + | [[Category: Vendruscolo M]] | ||
| + | [[Category: Voleti R]] | ||
| + | [[Category: Xu J]] | ||
| + | [[Category: Zhou A]] | ||
| + | [[Category: Zhou P]] | ||
Current revision
Dynamic binding mode of a synaptotagmin-1-SNARE complex in solution
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Categories: Homo sapiens | Large Structures | Rattus norvegicus | Bacaj T | Barlow N | Bonvin A | Brewer K | Camilloni C | Cavalli A | Graham B | Haussinger D | Liu J | Prinslow E | Rizo J | Seven A | Sudhof T | Swarbrick J | Tomchick D | Vendruscolo M | Voleti R | Xu J | Zhou A | Zhou P
