3j70
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Model of gp120, including variable regions, in complex with CD4 and 17b== | |
| + | <SX load='3j70' size='340' side='right' viewer='molstar' caption='[[3j70]], [[Resolution|resolution]] 20.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3j70]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J70 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 20Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j70 OCA], [https://pdbe.org/3j70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j70 RCSB], [https://www.ebi.ac.uk/pdbsum/3j70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j70 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CD4_HUMAN CD4_HUMAN] Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Envelope glycoprotein gp120 of HIV-1 possesses several variable regions; their precise structure has been difficult to establish. We report a new model of gp120, in complex with antibodies CD4 and 17b, complete with its variable regions. The model was produced by a computational protocol that uses cryo-electron microscopy (EM) maps, atomic-resolution structures of the core, and information on binding interactions. Our model has excellent fit with EMD: 5020, is stereochemically and energetically favorable, and has the expected binding interfaces. Comparison of the ternary arrangement of the loops in this model with those bound to PGT122 and PGV04 suggested a possible motion of the V1V2 away from the CCR5 binding site and toward CD4. Our study also revealed that the CD4-bound state of the V1V2 loop is not optimal for gp120 bound with several neutralizing antibodies. | ||
| - | + | Computational Refinement and Validation Protocol for Proteins with Large Variable Regions Applied to Model HIV Env Spike in CD4 and 17b Bound State.,Rasheed M, Bettadapura R, Bajaj C Structure. 2015 Jun 2;23(6):1138-49. doi: 10.1016/j.str.2015.03.026. PMID:26039348<ref>PMID:26039348</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 3j70" style="background-color:#fffaf0;"></div> |
| - | [[Category: Bettadapura | + | |
| - | [[Category: | + | ==See Also== |
| + | *[[CD4 3D structures|CD4 3D structures]] | ||
| + | *[[Gp120 3D structures|Gp120 3D structures]] | ||
| + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Human immunodeficiency virus 1]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bajaj C]] | ||
| + | [[Category: Bettadapura R]] | ||
| + | [[Category: Rasheed M]] | ||
Current revision
Model of gp120, including variable regions, in complex with CD4 and 17b
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