4zbh

<StructureSection load='4zbh' size='340' side='right' caption='[[4zbh]], [[Resolution|resolution]] 1.50&Aring;' scene=''>

<table><tr><td colspan='2'>[[4zbh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZBH FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zbh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zbh RCSB], [http://www.ebi.ac.uk/pdbsum/4zbh PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

Archaea employ the archaellum, a type IV pilus-like nanomachine, for swimming motility. In the crenarchaeon Sulfolobus acidocaldarius, the archaellum consists of seven proteins: FlaB/X/G/F/H/I/J. FlaF is conserved and essential for archaellum assembly but no FlaF structures exist. Here, we truncated the FlaF N terminus and solved 1.5-A and 1.65-A resolution crystal structures of this monotopic membrane protein. Structures revealed an N-terminal alpha-helix and an eight-strand beta-sandwich, immunoglobulin-like fold with striking similarity to S-layer proteins. Crystal structures, X-ray scattering, and mutational analyses suggest dimer assembly is needed for in vivo function. The sole cell envelope component of S. acidocaldarius is a paracrystalline S-layer, and FlaF specifically bound to S-layer protein, suggesting that its interaction domain is located in the pseudoperiplasm with its N-terminal helix in the membrane. From these data, FlaF may act as the previously unknown archaellum stator protein that anchors the rotating archaellum to the archaeal cell envelope.

 

FlaF Is a beta-Sandwich Protein that Anchors the Archaellum in the Archaeal Cell Envelope by Binding the S-Layer Protein.,Banerjee A, Tsai CL, Chaudhury P, Tripp P, Arvai AS, Ishida JP, Tainer JA, Albers SV Structure. 2015 Apr 3. pii: S0969-2126(15)00079-9. doi:, 10.1016/j.str.2015.03.001. PMID:25865246<ref>PMID:25865246</ref>

== References ==

[[Category: Arvai, A S]]

[[Category: Ishida, J P]]

[[Category: Tainer, J A]]

[[Category: Tsai, C L]]

[[Category: Immune system]]

[[Category: Immunoglobulin-like beta-sandwich]]

[[Category: Stator protein]]