Maltose-binding protein

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{{STRUCTURE_1n3w| PDB=1n3w | SIZE=400| SCENE= |right|CAPTION=Maltose-binding protein complex with maltose, [[1n3w]] }}
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<StructureSection load='1anf' size='350' side='right' caption='E. coli maltose-binding protein complex with maltose (PDB entry [[1anf]])' scene='47/471830/Cv/1'>
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== Function ==
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'''Maltose-binding protein''' (MBP) or '''ABC maltose transporter''' is part of the maltose/maltodextrin system in ''E. coli'' which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport<ref>PMID:10452611</ref>.
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'''Maltose-binding protein''' (MBP) or '''ABC maltose transporter''' is part of the maltose/maltodextrin system in ''E. coli'' which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport. MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in ''E. coli'' by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose.
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== Relevance ==
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MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in ''E. coli'' by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose<ref>PMID:21125392</ref>.
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== Structural highlights ==
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The <scene name='47/471830/Cv/5'>MBP sugar binding cleft is rich with protein-ligand hydrogen bonds</scene><ref>PMID:9309217</ref>. Water molecules are shown as red spheres.
==3D structures of maltose-binding protein==
==3D structures of maltose-binding protein==
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[[Maltose-binding protein 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*'''MBP'''
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**[[1llx]], [[1jw4]], [[1lls]], [[1omp]] - MBP<br />
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**[[3sey]], [[3sex]], [[3sew]], [[3sev]], [[3seu]], [[3set]], [[3ses]], [[3ser]], [[2v93]], [[1zkb]], [[1n3x]], [[1peb ]], [[1mbp]], [[1mpc]], [[3kjt]], [[1mdp]], [[1mdq]], [[1mbp]] - MBP (mutant)<br />
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**[[2klf]], [[2d21]] – MBP – NMR<br />
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**[[1ziu]] – MBP (mutant) + Ni<br />
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**[[1zjl]] – MBP (mutant) + Zn<br />
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**[[1zmg]] - MBP (mutant) + Cu<br />
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**[[4log]] - MBP /nuclear receptor<br />
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**[[4ogm]] - MBP /pilin<br />
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**[[4pe2]] - MBP /pilA1<br />
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**[[4khz]] - MBP MalK, MalF, MalG, MBP <br />
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**[[3fh6]] - MBP MalK, MalF, MalG <br />
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**[[2xd2]] – SpMBP – ''Streptococcus pneumoniae''
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*MBP complex with saccharide
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**[[1n3w]], [[1nl5]], [[1mpd]], [[1iud]], [[1jvx]], [[1jvy]] - MBP (mutant) + maltose<br />
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**[[1anf]], [[1jw5]], [[2xz3]] - MBP + maltose<br />
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**[[3mbp]] - MBP + maltotriose<br />
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**[[4mbp]] - MBP + maltotetrose<br />
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**[[1ez9]] - MBP + maltodextrin<br />
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**[[3hpi]] – MBP (mutant) + sucrose<br />
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**[[1lax]] - MBP (mutant) + glucose<br />
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**[[2h25]], [[1ezo]], [[1dmb]] - MBP + β-cyclodextrin<br />
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**[[1ezp]] - MBP (mutant) + β-cyclodextrin<br />
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**[[1fqa]], [[1fqb]], [[1fqc]], [[1fqd]] - MBP + maltotetraitol<br />
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**[[3puy]], [[3puz]] - MBP MalK, MalF, MalG, MBP + maltose + AMPPNP<br />
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**[[3rlf]] - MBP MalK, MalF, MalG, MBP + maltose + AMPPNP<br />
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**[[4jbw]] - MBP MalK, MalF, MalG + EIIAGLC<br />
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**[[3pv0]] - MBP MalK, MalF, MalG, MBP + maltose <br />
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**[[2r6g]], [[3puv]], [[3puw]], [[3pux]], [[4ki0]] - MBP MalK, MalF, MalG, MBP + maltose + nucleotide<br />
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**[[3ob4]] - MBP AraH2 (mutant) + maltotriose<br />
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**[[1elj]] – MBP + glucose – ''Pyrococcus furiosus''<br />
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**[[3jyr]] – MBP + α-acarbose – ''Salmonella enterica''<br />
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**[[2xd3]] – SpMBP + maltopentaose
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*MBP complex with protein
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**[[3pgf]] – MBP (mutant) + synthetic antigen binder<br />
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== References ==
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**[[2r6g]], [[4khz]], [[4ki0]] - MBP + maltose transporter complex<br />
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<references/>
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**[[3puv]], [[3puw]], [[3pux]] – MBP + maltose transporter complex + ADP <br />
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**[[3rlf]] - MBP + maltose transporter complex + Mg-AMPPNP<br />
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**[[1svx]] – MBP + ankyrin repeat protein OFF7<br />
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**[[1nmu]] - MBP + ribosomal protein L30<br />
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**[[1t0k]] - MBP + ribosomal protein L30 + RNA<br />
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**[[3rum]] – MBP + ristocetin<br />
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**[[4nuf]] - MBP /nuclear receptor + orphan nuclear receptor peptide<br />
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}}
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[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

E. coli maltose-binding protein complex with maltose (PDB entry 1anf)

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References

  1. ↑ Kapust RB, Waugh DS. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 1999 Aug;8(8):1668-74. PMID:10452611 doi:http://dx.doi.org/10.1110/ps.8.8.1668
  2. ↑ Sun P, Tropea JE, Waugh DS. Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner. Methods Mol Biol. 2011;705:259-74. doi: 10.1007/978-1-61737-967-3_16. PMID:21125392 doi:http://dx.doi.org/10.1007/978-1-61737-967-3_16
  3. ↑ Quiocho FA, Spurlino JC, Rodseth LE. Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor. Structure. 1997 Aug 15;5(8):997-1015. PMID:9309217

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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