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| | ==Solution structure of the PsIAA4 oligomerization domain reveals interaction modes for transcription factors in early auxin response== | | ==Solution structure of the PsIAA4 oligomerization domain reveals interaction modes for transcription factors in early auxin response== |
| - | <StructureSection load='2m1m' size='340' side='right' caption='[[2m1m]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2m1m' size='340' side='right'caption='[[2m1m]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2m1m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Garden_pea Garden pea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M1M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2m1m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M1M FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IAA4/5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3888 Garden pea])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m1m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m1m RCSB], [http://www.ebi.ac.uk/pdbsum/2m1m PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m1m OCA], [https://pdbe.org/2m1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m1m RCSB], [https://www.ebi.ac.uk/pdbsum/2m1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m1m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IAA4_PEA IAA4_PEA]] Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression (By similarity). | + | [https://www.uniprot.org/uniprot/IAA4_PEA IAA4_PEA] Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression (By similarity). |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The plant hormone auxin activates primary response genes by facilitating proteolytic removal of AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA)-inducible repressors, which directly bind to transcriptional AUXIN RESPONSE FACTORS (ARF). Most AUX/IAA and ARF proteins share highly conserved C-termini mediating homotypic and heterotypic interactions within and between both protein families. The high-resolution NMR structure of C-terminal domains III and IV of the AUX/IAA protein PsIAA4 from pea (Pisum sativum) revealed a globular ubiquitin-like beta-grasp fold with homologies to the Phox and Bem1p (PB1) domain. The PB1 domain of wild-type PsIAA4 features two distinct surface patches of oppositely charged amino acid residues, mediating front-to-back multimerization via electrostatic interactions. Mutations of conserved basic or acidic residues on either face suppressed PsIAA4 PB1 homo-oligomerization in vitro and confirmed directional interaction of full-length PsIAA4 in vivo (yeast two-hybrid system). Mixing of oppositely mutated PsIAA4 PB1 monomers enabled NMR mapping of the negatively charged interface of the reconstituted PsIAA4 PB1 homodimer variant, whose stoichiometry (1:1) and equilibrium binding constant (KD approximately 6.4 muM) were determined by isothermal titration calorimetry. In silico protein-protein docking studies based on NMR and yeast interaction data derived a model of the PsIAA4 PB1 homodimer, which is comparable with other PB1 domain dimers, but indicated considerable differences between the homodimeric interfaces of AUX/IAA and ARF PB1 domains. Our study provides an impetus for elucidating the molecular determinants that confer specificity to complex protein-protein interaction circuits between members of the two central families of transcription factors important to the regulation of auxin-responsive gene expression.
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| - | Solution structure of the PsIAA4 oligomerization domain reveals interaction modes for transcription factors in early auxin response.,Dinesh DC, Kovermann M, Gopalswamy M, Hellmuth A, Calderon Villalobos LI, Lilie H, Balbach J, Abel S Proc Natl Acad Sci U S A. 2015 Apr 27. pii: 201424077. PMID:25918389<ref>PMID:25918389</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Garden pea]] | + | [[Category: Large Structures]] |
| - | [[Category: Abel, S]] | + | [[Category: Pisum sativum]] |
| - | [[Category: Balbach, J]] | + | [[Category: Abel S]] |
| - | [[Category: Dinesh, D C]] | + | [[Category: Balbach J]] |
| - | [[Category: Gopalswamy, M]] | + | [[Category: Dinesh DC]] |
| - | [[Category: Kovermann, M]] | + | [[Category: Gopalswamy M]] |
| - | [[Category: Transcription]]
| + | [[Category: Kovermann M]] |
