4y3i
From Proteopedia
(Difference between revisions)
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, low dose== | |
| + | <StructureSection load='4y3i' size='340' side='right'caption='[[4y3i]], [[Resolution|resolution]] 1.69Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4y3i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y3I FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LBV:3-[2-[(Z)-[3-(2-CARBOXYETHYL)-5-[(Z)-(4-ETHENYL-3-METHYL-5-OXIDANYLIDENE-PYRROL-2-YLIDENE)METHYL]-4-METHYL-PYRROL-1-IUM-2-YLIDENE]METHYL]-5-[(Z)-[(3E)-3-ETHYLIDENE-4-METHYL-5-OXIDANYLIDENE-PYRROLIDIN-2-YLIDENE]METHYL]-4-METHYL-1H-PYRROL-3-YL]PROPANOIC+ACID'>LBV</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y3i OCA], [https://pdbe.org/4y3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y3i RCSB], [https://www.ebi.ac.uk/pdbsum/4y3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y3i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/BPHY_DEIRA BPHY_DEIRA] Photoreceptor which exists in two forms that are reversibly interconvertible by light: the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region. Has also a slight blue shift for the far-red maximum. Could also absorb green light. May participate in regulating pigment synthesis like the carotenoid deinoxanthin which could protect the bacterium from intense visible light. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins. | ||
| - | + | X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. radiodurans phytochrome.,Li F, Burgie ES, Yu T, Heroux A, Schatz GC, Vierstra RD, Orville AM J Am Chem Soc. 2015 Mar 4;137(8):2792-5. doi: 10.1021/ja510923m. Epub 2015 Feb, 18. PMID:25650486<ref>PMID:25650486</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4y3i" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Li | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Deinococcus radiodurans R1]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| + | [[Category: Burgie ES]] | ||
| + | [[Category: Heroux A]] | ||
| + | [[Category: Li F]] | ||
| + | [[Category: Orville AM]] | ||
| + | [[Category: Schatz GC]] | ||
| + | [[Category: Vierstra RD]] | ||
| + | [[Category: Yu T]] | ||
Current revision
PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, low dose
| |||||||||||
