2z4h

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[[Image:2z4h.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the Cpx pathway activator NlpE from Escherichia coli==
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|PDB= 2z4h |SIZE=350|CAPTION= <scene name='initialview01'>2z4h</scene>, resolution 2.80&Aring;
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<StructureSection load='2z4h' size='340' side='right'caption='[[2z4h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[2z4h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z4H FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE= nlpE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4h OCA], [https://pdbe.org/2z4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z4h RCSB], [https://www.ebi.ac.uk/pdbsum/2z4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NLPE_ECOLI NLPE_ECOLI] Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR.<ref>PMID:15252048</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z4h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z4h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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NlpE, an outer membrane lipoprotein, functions during envelope stress responses in Gram-negative bacteria. In Escherichia coli, adhesion to abiotic surfaces has been reported to activate the Cpx pathway in an NlpE-dependent manner. External copper ions are also thought to activate the Cpx pathway mediated by NlpE. We determined the crystal structure of NlpE from E. coli at 2.6 A resolution. The structure showed that NlpE consists of two beta barrel domains. The N-terminal domain resembles the bacterial lipocalin Blc, and the C-terminal domain has an oligonucleotide/oligosaccharide-binding (OB) fold. From both biochemical analyses and the crystal structure, it can be deduced that the cysteine residues in the CXXC motif may be chemically active. Furthermore, two monomers in the asymmetric unit form an unusual 3D domain-swapped dimer. These findings indicate that tertiary and/or quaternary structural instability may be responsible for Cpx pathway activation.
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'''Crystal structure of the Cpx pathway activator NlpE from Escherichia coli'''
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Structural studies of the Cpx pathway activator NlpE on the outer membrane of Escherichia coli.,Hirano Y, Hossain MM, Takeda K, Tokuda H, Miki K Structure. 2007 Aug;15(8):963-76. PMID:17698001<ref>PMID:17698001</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2z4h" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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NlpE, an outer membrane lipoprotein, functions during envelope stress responses in Gram-negative bacteria. In Escherichia coli, adhesion to abiotic surfaces has been reported to activate the Cpx pathway in an NlpE-dependent manner. External copper ions are also thought to activate the Cpx pathway mediated by NlpE. We determined the crystal structure of NlpE from E. coli at 2.6 A resolution. The structure showed that NlpE consists of two beta barrel domains. The N-terminal domain resembles the bacterial lipocalin Blc, and the C-terminal domain has an oligonucleotide/oligosaccharide-binding (OB) fold. From both biochemical analyses and the crystal structure, it can be deduced that the cysteine residues in the CXXC motif may be chemically active. Furthermore, two monomers in the asymmetric unit form an unusual 3D domain-swapped dimer. These findings indicate that tertiary and/or quaternary structural instability may be responsible for Cpx pathway activation.
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*[[Copper homeostasis protein|Copper homeostasis protein]]
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== References ==
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==About this Structure==
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<references/>
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2Z4H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4H OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structural studies of the Cpx pathway activator NlpE on the outer membrane of Escherichia coli., Hirano Y, Hossain MM, Takeda K, Tokuda H, Miki K, Structure. 2007 Aug;15(8):963-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17698001 17698001]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hirano, Y.]]
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[[Category: Hirano Y]]
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[[Category: Hossain, M M.]]
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[[Category: Hossain MM]]
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[[Category: Miki, K.]]
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[[Category: Miki K]]
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[[Category: Takeda, K.]]
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[[Category: Takeda K]]
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[[Category: Tokuda, H.]]
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[[Category: Tokuda H]]
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[[Category: SO4]]
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[[Category: 3d domain swapping]]
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[[Category: beta barrel]]
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[[Category: ob-fold]]
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[[Category: outer memblane lipoprotein]]
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[[Category: signaling protein activator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:52:06 2008''
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Current revision

Crystal structure of the Cpx pathway activator NlpE from Escherichia coli

PDB ID 2z4h

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