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2z8m

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[[Image:2z8m.jpg|left|200px]]
 
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{{Structure
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==Structural basis for the catalytic mechanism of phosphothreonine lyase==
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|PDB= 2z8m |SIZE=350|CAPTION= <scene name='initialview01'>2z8m</scene>, resolution 2.0&Aring;
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<StructureSection load='2z8m' size='340' side='right'caption='[[2z8m]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2z8m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8M FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= mkaD, spvC, vsdD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8m OCA], [https://pdbe.org/2z8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8m RCSB], [https://www.ebi.ac.uk/pdbsum/2z8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8m ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPVC_SALTY SPVC_SALTY] Secreted effector that irreversibly inactivates host MAP kinases by catalyzing the dephosphorylation of the phosphothreonine residue in the pT-X-pY motif in MAPK2/ERK2, MAPK3/ERK1, and p38, via a beta-elimination reaction leading to a dehydrobutyrine residue. Is also able to remove the phosphate group from phospho-JNK in vitro, but JNK may not be a substrate in vivo. Could help suppress localized proinflammatory responses at infection foci in the spleen and liver, and thereby facilitate bacterial growth.<ref>PMID:17303758</ref> <ref>PMID:18060821</ref> <ref>PMID:18084305</ref> <ref>PMID:18284579</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Salmonella SpvC belongs to a new enzyme family designated phosphothreonine lyases that irreversibly inactivate mitogen-activated protein kinases. The crystal structure of SpvC reported here reveals that the two phosphorylated residues in the substrate peptide predominantly mediate its recognition by SpvC. Substrate-induced conformational changes in SpvC sequester the phosphothreonine in a completely solvent-free environment, preventing the hydrolysis of the phosphate group and facilitating the elimination reaction.
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'''Structural basis for the catalytic mechanism of phosphothreonine lyase'''
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Structural basis for the catalytic mechanism of phosphothreonine lyase.,Chen L, Wang H, Zhang J, Gu L, Huang N, Zhou JM, Chai J Nat Struct Mol Biol. 2008 Jan;15(1):101-2. Epub 2007 Dec 16. PMID:18084305<ref>PMID:18084305</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2Z8M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8M OCA].
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<div class="pdbe-citations 2z8m" style="background-color:#fffaf0;"></div>
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[[Category: Salmonella typhimurium]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Chai, J J.]]
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__TOC__
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[[Category: Chen, L J.]]
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</StructureSection>
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[[Category: Gu, L C.]]
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[[Category: Large Structures]]
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[[Category: Huang, N.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Wang, H Y.]]
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[[Category: Chai J]]
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[[Category: Zhou, J M.]]
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[[Category: Chen L]]
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[[Category: distorted beta-strand sheet]]
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[[Category: Gu L]]
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[[Category: lyase]]
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[[Category: Huang N]]
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[[Category: plasmid]]
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[[Category: Wang H]]
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[[Category: short three-helix bundle]]
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[[Category: Zhou JM]]
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[[Category: virulence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:52:55 2008''
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Current revision

Structural basis for the catalytic mechanism of phosphothreonine lyase

PDB ID 2z8m

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