4rsl

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==Structure of fructosyl peptide oxidase from E. terrenum==
==Structure of fructosyl peptide oxidase from E. terrenum==
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<StructureSection load='4rsl' size='340' side='right' caption='[[4rsl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='4rsl' size='340' side='right'caption='[[4rsl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4rsl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RSL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RSL FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4rsl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_terrenum Penicillium terrenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RSL FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rsl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rsl RCSB], [http://www.ebi.ac.uk/pdbsum/4rsl PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rsl OCA], [https://pdbe.org/4rsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rsl RCSB], [https://www.ebi.ac.uk/pdbsum/4rsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rsl ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q765A9_9EURO Q765A9_9EURO]
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The FAOD/FPOD family of proteins has the potential to be useful for the longterm detection of blood glucose levels in diabetes patients. A bottleneck for this application is to find or engineer a FAOD/FPOD family enzyme that is specifically active towards alpha-fructosyl peptides but is inactive towards other types of glycated peptides. Here, the crystal structure of fructosyl peptide oxidase from Eupenicillium terrenum (EtFPOX) is reported at 1.9 A resolution. In contrast to the previously reported structure of amadoriase II, EtFPOX has an open substrate entrance to accommodate the large peptide substrate. The functions of residues critical for substrate selection are discussed based on structure comparison and sequence alignment. This study reveals the first structural details of group I FPODs that prefer alpha-fructosyl substrates and could provide significant useful information for uncovering the mechanism of substrate specificity of FAOD/FPODs and guidance towards future enzyme engineering for diagnostic purposes.
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Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum.,Gan W, Gao F, Xing K, Jia M, Liu H, Gong W Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):381-7. doi:, 10.1107/S2053230X15003921. Epub 2015 Mar 20. PMID:25849495<ref>PMID:25849495</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Gan, W]]
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[[Category: Large Structures]]
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[[Category: Gao, F]]
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[[Category: Penicillium terrenum]]
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[[Category: Gong, W]]
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[[Category: Gan W]]
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[[Category: Jia, M]]
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[[Category: Gao F]]
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[[Category: Liu, H]]
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[[Category: Gong W]]
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[[Category: Xing, K]]
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[[Category: Jia M]]
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[[Category: Fad]]
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[[Category: Liu H]]
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[[Category: Oxidoreductase]]
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[[Category: Xing K]]

Current revision

Structure of fructosyl peptide oxidase from E. terrenum

PDB ID 4rsl

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