2n0r
From Proteopedia
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==RNA structure determination by solid-state NMR spectroscopy== | ==RNA structure determination by solid-state NMR spectroscopy== | ||
| - | <StructureSection load='2n0r' size='340' side='right' caption='[[2n0r | + | <StructureSection load='2n0r' size='340' side='right'caption='[[2n0r]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2n0r]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N0R OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[2n0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N0R FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n0r OCA], [https://pdbe.org/2n0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n0r RCSB], [https://www.ebi.ac.uk/pdbsum/2n0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n0r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines-independent of their ability to crystallize- and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies. | ||
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| + | RNA structure determination by solid-state NMR spectroscopy.,Marchanka A, Simon B, Althoff-Ospelt G, Carlomagno T Nat Commun. 2015 May 11;6:7024. doi: 10.1038/ncomms8024. PMID:25960310<ref>PMID:25960310</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2n0r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Althoff-Ospelt | + | [[Category: Large Structures]] |
| - | [[Category: Carlomagno | + | [[Category: Synthetic construct]] |
| - | [[Category: Marchanka | + | [[Category: Althoff-Ospelt G]] |
| - | [[Category: Simon | + | [[Category: Carlomagno T]] |
| - | + | [[Category: Marchanka A]] | |
| + | [[Category: Simon B]] | ||
Current revision
RNA structure determination by solid-state NMR spectroscopy
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