2n2y
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2n2y is ON HOLD Authors: Williams Jr., D.C., Walavalkar, N.M., Buchwald, W.A. Description: Solution structure of the meiosis-expressed gene 1 (Meig...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of the meiosis-expressed gene 1 (Meig1)== | |
| + | <StructureSection load='2n2y' size='340' side='right'caption='[[2n2y]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2n2y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N2Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n2y OCA], [https://pdbe.org/2n2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n2y RCSB], [https://www.ebi.ac.uk/pdbsum/2n2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n2y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MEIG1_MOUSE MEIG1_MOUSE] Essential for spermiogenesis.<ref>PMID:1390336</ref> <ref>PMID:19805151</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show that MEIG1 adopts a unique fold that provides a large surface for interacting with other proteins. We mutated 12 exposed and conserved amino acids and show that four of these mutations (W50A, K57E, F66A, Y68A) dramatically reduce binding to PACRG. These four amino acids form a contiguous hydrophobic patch on one end of the protein. Furthermore, each of these four mutations diminishes the ability of MEIG1 to stabilize PACRG when expressed in bacteria. Together these studies establish the unique structure and key interaction surface of MEIG1 and provide a framework to explore how MEIG1 recruits proteins to build the sperm tail. | ||
| - | + | Dissecting the structural basis of MEIG1 interaction with PACRG.,Li W, Walavalkar NM, Buchwald WA, Teves ME, Zhang L, Liu H, Bilinovich S, Peterson DL, Strauss Iii JF, Williams DC Jr, Zhang Z Sci Rep. 2016 Jan 4;6:18278. doi: 10.1038/srep18278. PMID:26726850<ref>PMID:26726850</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 2n2y" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Williams Jr]] | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Buchwald WA]] | ||
| + | [[Category: Walavalkar NM]] | ||
| + | [[Category: Williams Jr DC]] | ||
Current revision
Solution structure of the meiosis-expressed gene 1 (Meig1)
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