349d

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[[Image:349d.gif|left|200px]]
 
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{{Structure
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==X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: HIGH SALT CONCENTRATION==
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|PDB= 349d |SIZE=350|CAPTION= <scene name='initialview01'>349d</scene>, resolution 1.900&Aring;
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<StructureSection load='349d' size='340' side='right'caption='[[349d]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[349d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=349D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=349D FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=349d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=349d OCA], [https://pdbe.org/349d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=349d RCSB], [https://www.ebi.ac.uk/pdbsum/349d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=349d ProSAT]</span></td></tr>
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}}
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</table>
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__TOC__
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'''X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: HIGH SALT CONCENTRATION'''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Finley JB]]
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==Overview==
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[[Category: Luo M]]
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The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing half the human papilloma virus E2 binding site, has been solved from two crystals grown at different ionic conditions (50 mM MgCl2and 50 mM spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in salt concentration, the two DNA structures are in a very similar, A-type DNA conformation, with helical axes curving towards the major groove. Although the salt concentrations do not effect the helical parameters or hydration to a large degree, there is a change in the overall helical curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and biologically relevant when compared with similar DNA structures, including the E2 binding site of a protein-DNA complex.
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==About this Structure==
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349D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=349D OCA].
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==Reference==
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X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9838004 9838004]
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[[Category: Protein complex]]
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[[Category: Finley, J B.]]
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[[Category: Luo, M.]]
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[[Category: a-dna]]
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[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:54:53 2008''
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Current revision

X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: HIGH SALT CONCENTRATION

PDB ID 349d

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