4zsz
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-3.0== | |
| + | <StructureSection load='4zsz' size='340' side='right'caption='[[4zsz]], [[Resolution|resolution]] 4.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4zsz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZSZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.251Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zsz OCA], [https://pdbe.org/4zsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zsz RCSB], [https://www.ebi.ac.uk/pdbsum/4zsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zsz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O67778_AQUAE O67778_AQUAE] [https://www.uniprot.org/uniprot/Q9I208_PSEAE Q9I208_PSEAE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Connecting proteins together in prescribed geometric arrangements is an important element in new areas of biomolecular design. In this study, we characterize the degree of three-dimensional orientational control that can be achieved when two protein domains that have alpha-helical termini are joined using an alpha-helical linker. A fusion between naturally oligomeric protein domains was designed in this fashion with the intent of creating a self-assembling 12-subunit tetrahedral protein cage. While the designed fusion protein failed to assemble into a tetrahedral cage in high yield, a series of crystal structures showed that the two fused components were indeed bridged by an intact alpha helix, although the fusion protein was distorted from the intended ideal configuration by bending of the helix, ranging from 7 to 35 degrees . That range of deviation in orientation creates challenges for designing large, perfectly symmetric protein assemblies, although it should offer useful outcomes for other less geometrically demanding applications in synthetic biology. | ||
| - | + | On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.,Lai YT, Jiang L, Chen W, Yeates TO Protein Eng Des Sel. 2015 Aug 4. pii: gzv035. PMID:26243886<ref>PMID:26243886</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4zsz" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lai | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aquifex aeolicus VF5]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
| + | [[Category: Lai Y-T]] | ||
| + | [[Category: Yeates TO]] | ||
Current revision
Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-3.0
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