378d

[[Image:378d.gif|left|200px]]

|PDB= 378d |SIZE=350|CAPTION= <scene name='initialview01'>378d</scene>, resolution 2.400&Aring;

|SITE=

|LIGAND= <scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>

|ACTIVITY=

 

'''STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA'''

 

 

==Overview==

The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [&gt;/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.

 

378D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA].

 

Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089456 10089456]

[[Category: Mitra, S N.]]

[[Category: Sundaralingam, M.]]

[[Category: Wahl, M C.]]

[[Category: NA]]

[[Category: double drug in minor groove]]

[[Category: double helix]]

[[Category: right handed dna]]