This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


398d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:39, 7 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:398d.gif|left|200px]]
 
-
{{Structure
+
==3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION==
-
|PDB= 398d |SIZE=350|CAPTION= <scene name='initialview01'>398d</scene>, resolution 1.940&Aring;
+
<StructureSection load='398d' size='340' side='right'caption='[[398d]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[398d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=398D FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=398d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=398d OCA], [https://pdbe.org/398d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=398d RCSB], [https://www.ebi.ac.uk/pdbsum/398d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=398d ProSAT]</span></td></tr>
-
}}
+
</table>
-
 
+
__TOC__
-
'''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''
+
</StructureSection>
-
 
+
[[Category: Large Structures]]
-
 
+
[[Category: Cellai L]]
-
==Overview==
+
[[Category: Heumann H]]
-
During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.
+
[[Category: Meier G]]
-
 
+
[[Category: Mochi-Onori A]]
-
==About this Structure==
+
[[Category: Mueller U]]
-
398D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication., Mueller U, Maier G, Mochi Onori A, Cellai L, Heumann H, Heinemann U, Biochemistry. 1998 Sep 1;37(35):12005-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9724510 9724510]
+
-
[[Category: Protein complex]]
+
-
[[Category: Cellai, L.]]
+
-
[[Category: Heumann, H.]]
+
-
[[Category: Meier, G.]]
+
-
[[Category: Mochi-Onori, A.]]
+
-
[[Category: Mueller, U.]]
+
-
[[Category: a-dna/rna]]
+
-
[[Category: double helix]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:19 2008''
+

Current revision

3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION

PDB ID 398d

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/398d"
Personal tools