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4zej

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==Crystal Structure of DIM-1 Metallo-beta-Lactamase exposed to Ceftazidime==
==Crystal Structure of DIM-1 Metallo-beta-Lactamase exposed to Ceftazidime==
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<StructureSection load='4zej' size='340' side='right' caption='[[4zej]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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<StructureSection load='4zej' size='340' side='right'caption='[[4zej]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4zej]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZEJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZEJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4zej]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZEJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wd6|4wd6]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zej OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zej RCSB], [http://www.ebi.ac.uk/pdbsum/4zej PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zej OCA], [https://pdbe.org/4zej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zej RCSB], [https://www.ebi.ac.uk/pdbsum/4zej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zej ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/D5JGF6_STUST D5JGF6_STUST]
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A carbapenem-resistant Pseudomonas stutzeri strain isolated from a Dutch patient was analyzed in detail. This isolate produced a metallo-beta-lactamase (MBL) whose gene, with 43.5% GC content, was cloned and expressed in Escherichia coli. beta-Lactamase DIM-1 (for Dutch imipenemase) was weakly related to other Ambler class B beta-lactamases, sharing &lt;52% amino acid identity with the most closely related MBL, GIM-1, and 45% identity with IMP-type MBLs. The beta-Lactamase DIM-1 significantly hydrolyzed broad-spectrum cephalosporins and carbapenems and spared aztreonam. This MBL gene was embedded in a class 1 integron containing two other gene cassettes, encoding resistance to aminoglycosides and disinfectants, that was located on a 70-kb plasmid.
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Characterization of DIM-1, an integron-encoded metallo-beta-lactamase from a Pseudomonas stutzeri clinical isolate in the Netherlands.,Poirel L, Rodriguez-Martinez JM, Al Naiemi N, Debets-Ossenkopp YJ, Nordmann P Antimicrob Agents Chemother. 2010 Jun;54(6):2420-4. doi: 10.1128/AAC.01456-09., Epub 2010 Mar 22. PMID:20308383<ref>PMID:20308383</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Booth, M P.S]]
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[[Category: Large Structures]]
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[[Category: Kosmopoulou, M]]
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[[Category: Pseudomonas stutzeri]]
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[[Category: Spencer, J]]
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[[Category: Booth MPS]]
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[[Category: Hydrolase]]
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[[Category: Kosmopoulou M]]
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[[Category: Zinc metalloenzyme]]
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[[Category: Spencer J]]

Current revision

Crystal Structure of DIM-1 Metallo-beta-Lactamase exposed to Ceftazidime

PDB ID 4zej

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