3b5x

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[[Image:3b5x.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of MsbA from Vibrio cholerae==
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|PDB= 3b5x |SIZE=350|CAPTION= <scene name='initialview01'>3b5x</scene>, resolution 5.500&Aring;
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<StructureSection load='3b5x' size='340' side='right'caption='[[3b5x]], [[Resolution|resolution]] 5.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[3b5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B5X FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.5&#8491;</td></tr>
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|GENE= msbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5x OCA], [https://pdbe.org/3b5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b5x RCSB], [https://www.ebi.ac.uk/pdbsum/3b5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5x ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Crystal Structure of MsbA from Vibrio cholerae'''
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[https://www.uniprot.org/uniprot/MSBA_VIBCH MSBA_VIBCH] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-ray structures of the bacterial ABC lipid flippase, MsbA, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotide-free conformations of MsbA reveals a flexible hinge formed by extracellular loops 2 and 3. This hinge allows the nucleotide-binding domains to disassociate while the ATP-binding half sites remain facing each other. The binding of the nucleotide causes a packing rearrangement of the transmembrane helices and changes the accessibility of the transporter from cytoplasmic (inward) facing to extracellular (outward) facing. The inward and outward openings are mediated by two different sets of transmembrane helix interactions. Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/3b5x_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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3B5X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5X OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b5x ConSurf].
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Flexibility in the ABC transporter MsbA: Alternating access with a twist., Ward A, Reyes CL, Yu J, Roth CB, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19005-10. Epub 2007 Nov 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18024585 18024585]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Chang, G.]]
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[[Category: Chang G]]
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[[Category: Reyes, C L.]]
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[[Category: Reyes CL]]
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[[Category: Roth, C B.]]
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[[Category: Roth CB]]
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[[Category: Ward, A.]]
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[[Category: Ward A]]
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[[Category: Yu, J.]]
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[[Category: Yu J]]
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[[Category: abc transporter]]
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[[Category: atp-binding]]
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[[Category: hydrolase]]
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[[Category: inner membrane]]
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[[Category: lipid flippase]]
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[[Category: lipid transport]]
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[[Category: membrane]]
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[[Category: membrane protein]]
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[[Category: msba]]
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[[Category: nucleotide-binding]]
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[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:56:21 2008''
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Current revision

Crystal Structure of MsbA from Vibrio cholerae

PDB ID 3b5x

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