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5a3e
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==2.5A structure of lysozyme determined by MicroED with data from a single crystal== | |
| + | <StructureSection load='5a3e' size='340' side='right'caption='[[5a3e]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5a3e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A3E FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2.501Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a3e OCA], [https://pdbe.org/5a3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a3e RCSB], [https://www.ebi.ac.uk/pdbsum/5a3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a3e ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology. | ||
| - | + | High-resolution structure determination by continuous-rotation data collection in MicroED.,Nannenga BL, Shi D, Leslie AG, Gonen T Nat Methods. 2014 Aug 3. doi: 10.1038/nmeth.3043. PMID:25086503<ref>PMID:25086503</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5a3e" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Nannenga | + | ==See Also== |
| - | [[Category: | + | *[[Lysozyme 3D structures|Lysozyme 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gallus gallus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gonen T]] | ||
| + | [[Category: Leslie AGW]] | ||
| + | [[Category: Nannenga BL]] | ||
| + | [[Category: Shi D]] | ||
Current revision
2.5A structure of lysozyme determined by MicroED with data from a single crystal
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