4ycr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:31, 9 May 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Structure determination of an integral membrane protein at room temperature from crystals in situ==
==Structure determination of an integral membrane protein at room temperature from crystals in situ==
-
<StructureSection load='4ycr' size='340' side='right' caption='[[4ycr]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+
<StructureSection load='4ycr' size='340' side='right'caption='[[4ycr]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4ycr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YCR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YCR FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4ycr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YCR FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m71|3m71]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ycr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ycr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ycr RCSB], [http://www.ebi.ac.uk/pdbsum/4ycr PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ycr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ycr OCA], [https://pdbe.org/4ycr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ycr RCSB], [https://www.ebi.ac.uk/pdbsum/4ycr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ycr ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/TEHA_HAEIN TEHA_HAEIN]] Ion channel involved in potassium tellurite resistance.
+
[https://www.uniprot.org/uniprot/TEHA_HAEIN TEHA_HAEIN] Ion channel involved in potassium tellurite resistance.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystals in situ eliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination of Haemophilus influenza TehA at 2.3 A resolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines.
 +
 
 +
Structure determination of an integral membrane protein at room temperature from crystals in situ.,Axford D, Foadi J, Hu NJ, Choudhury HG, Iwata S, Beis K, Evans G, Alguel Y Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1228-37. doi:, 10.1107/S139900471500423X. Epub 2015 May 14. PMID:26057664<ref>PMID:26057664</ref>
 +
 
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 4ycr" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Alguel, Y]]
+
[[Category: Haemophilus influenzae Rd KW20]]
-
[[Category: Axford, D]]
+
[[Category: Large Structures]]
-
[[Category: Beis, K]]
+
[[Category: Alguel Y]]
-
[[Category: Choudhury, H G]]
+
[[Category: Axford D]]
-
[[Category: Evans, G]]
+
[[Category: Beis K]]
-
[[Category: Foadi, J]]
+
[[Category: Choudhury HG]]
-
[[Category: Hu, N J]]
+
[[Category: Evans G]]
-
[[Category: Iwata, S]]
+
[[Category: Foadi J]]
-
[[Category: In situ data collection]]
+
[[Category: Hu NJ]]
-
[[Category: Membrane protein]]
+
[[Category: Iwata S]]
-
[[Category: Multiple dataset]]
+
-
[[Category: Synchrotron beamline]]
+

Current revision

Structure determination of an integral membrane protein at room temperature from crystals in situ

PDB ID 4ycr

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ycr"
Personal tools