5a2j

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==Crystal structure of scFv-SM3 in complex with the naked peptide APDTRP==
==Crystal structure of scFv-SM3 in complex with the naked peptide APDTRP==
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<StructureSection load='5a2j' size='340' side='right' caption='[[5a2j]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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<StructureSection load='5a2j' size='340' side='right'caption='[[5a2j]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5a2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A2J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5a2j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A2J FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a2i|5a2i]], [[5a2k|5a2k]], [[5a2l|5a2l]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5a2j RCSB], [http://www.ebi.ac.uk/pdbsum/5a2j PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2j OCA], [https://pdbe.org/5a2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a2j RCSB], [https://www.ebi.ac.uk/pdbsum/5a2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a2j ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HVM32_MOUSE HVM32_MOUSE] [https://www.uniprot.org/uniprot/LV1E_MOUSE LV1E_MOUSE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structural features of MUC1-like glycopeptides bearing the Tn antigen (alpha-O-GalNAc-Ser/Thr) in complex with an anti MUC-1 antibody are reported at atomic resolution. For the alpha-O-GalNAc-Ser derivative, the glycosidic linkage adopts a high-energy conformation, barely populated in the free state. This unusual structure (also observed in an alpha-S-GalNAc-Cys mimic) is stabilized by hydrogen bonds between the peptidic fragment and the sugar. The selection of a particular peptide structure by the antibody is thus propagated to the carbohydrate through carbohydrate/peptide contacts, which force a change in the orientation of the sugar moiety. This seems to be unfeasible in the alpha-O-GalNAc-Thr glycopeptide owing to the more limited flexibility of the side chain imposed by the methyl group. Our data demonstrate the non-equivalence of Ser and Thr O-glycosylation points in molecular recognition processes. These features provide insight into the occurrence in nature of the APDTRP epitope for anti-MUC1 antibodies.
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Deciphering the Non-Equivalence of Serine and Threonine O-Glycosylation Points: Implications for Molecular Recognition of the Tn Antigen by an anti-MUC1 Antibody.,Martinez-Saez N, Castro-Lopez J, Valero-Gonzalez J, Madariaga D, Companon I, Somovilla VJ, Salvado M, Asensio JL, Jimenez-Barbero J, Avenoza A, Busto JH, Bernardes GJ, Peregrina JM, Hurtado-Guerrero R, Corzana F Angew Chem Int Ed Engl. 2015 Jun 26. doi: 10.1002/anie.201502813. PMID:26118689<ref>PMID:26118689</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5a2j" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Asensio, J L]]
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[[Category: Homo sapiens]]
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[[Category: Avenoza, A]]
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[[Category: Large Structures]]
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[[Category: Bernardes, G J.L]]
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[[Category: Mus musculus]]
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[[Category: Busto, J H]]
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[[Category: Asensio JL]]
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[[Category: Castro-Lopez, J]]
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[[Category: Avenoza A]]
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[[Category: Companon, I]]
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[[Category: Bernardes GJL]]
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[[Category: Corzana, F]]
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[[Category: Busto JH]]
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[[Category: Hurtado-Guerrero, R]]
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[[Category: Castro-Lopez J]]
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[[Category: Jimenez-Barbero, J]]
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[[Category: Companon I]]
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[[Category: Madariaga, D]]
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[[Category: Corzana F]]
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[[Category: Martinez-Saez, N]]
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[[Category: Hurtado-Guerrero R]]
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[[Category: Peregrina, J M]]
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[[Category: Jimenez-Barbero J]]
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[[Category: Salvado, M]]
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[[Category: Madariaga D]]
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[[Category: Somovilla, V J]]
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[[Category: Martinez-Saez N]]
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[[Category: Valero-Gonzalez, J]]
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[[Category: Peregrina JM]]
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[[Category: Antibody]]
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[[Category: Salvado M]]
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[[Category: Conformation analysis]]
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[[Category: Somovilla VJ]]
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[[Category: Fusion protein]]
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[[Category: Valero-Gonzalez J]]
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[[Category: Glycopeptide]]
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[[Category: Molecular recognition]]
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[[Category: Peptide binding protein]]
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Current revision

Crystal structure of scFv-SM3 in complex with the naked peptide APDTRP

PDB ID 5a2j

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