5al7
From Proteopedia
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==N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.== | ==N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.== | ||
| - | <StructureSection load='5al7' size='340' side='right' caption='[[5al7]], [[Resolution|resolution]] 2.92Å' scene=''> | + | <StructureSection load='5al7' size='340' side='right'caption='[[5al7]], [[Resolution|resolution]] 2.92Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5al7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AL7 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5al7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AL7 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5al7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5al7 OCA], [https://pdbe.org/5al7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5al7 RCSB], [https://www.ebi.ac.uk/pdbsum/5al7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5al7 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/SAS6_DROME SAS6_DROME] Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication.<ref>PMID:17412918</ref> <ref>PMID:17463247</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the nine-fold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved, but they all contain a predicted Central Coiled-Coil Domain (CCCD). Here we show that the Drosophila Ana2 CCCD forms a tetramer, and we solve its structure to 0.8 A, revealing that it adopts an unusual parallel-coil topology. We also solve the structure of the Drosophila Sas-6 N-terminal domain to 2.9 A revealing that it forms higher-order oligomers through canonical interactions. Point mutations that perturb Sas-6 or Ana2 homo-oligomerisation in vitro strongly perturb centriole assembly in vivo. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2, and the Ana2 CCCD tetramer structure provides important information on how these proteins might cooperate to form a cartwheel structure. | ||
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| + | The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.,Cottee MA, Muschalik N, Johnson S, Leveson J, Raff JW, Lea SM Elife. 2015 May 23;4. doi: 10.7554/eLife.07236. PMID:26002084<ref>PMID:26002084</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5al7" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Cottee MA]] |
| - | [[Category: | + | [[Category: Johnson S]] |
| - | [[Category: | + | [[Category: Lea SM]] |
| - | + | ||
Current revision
N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.
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