3bsn

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[[Image:3bsn.jpg|left|200px]]
 
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{{Structure
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==Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA==
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|PDB= 3bsn |SIZE=350|CAPTION= <scene name='initialview01'>3bsn</scene>, resolution 1.80&Aring;
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<StructureSection load='3bsn' size='340' side='right'caption='[[3bsn]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=N5C:'>N5C</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[3bsn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSN FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=N5C:5-NITROCYTIDINE+5-(TETRAHYDROGEN+TRIPHOSPHATE)'>N5C</scene>, <scene name='pdbligand=N5M:5-NITROCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>N5M</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsn OCA], [https://pdbe.org/3bsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsn RCSB], [https://www.ebi.ac.uk/pdbsum/3bsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q70ET3_9CALI Q70ET3_9CALI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bsn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bsn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of Norwalk virus polymerase bound to an RNA primer-template duplex and either the natural substrate CTP or the inhibitor 5-nitrocytidine triphosphate have been determined to 1.8A resolution. These structures reveal a closed conformation of the polymerase that differs significantly from previously determined open structures of calicivirus and picornavirus polymerases. These closed complexes are trapped immediately prior to the nucleotidyl transfer reaction, with the triphosphate group of the nucleotide bound to two manganese ions at the active site, poised for reaction to the 3'-hydroxyl group of the RNA primer. The positioning of the 5-nitrocytidine triphosphate nitro group between the alpha-phosphate and the 3'-hydroxyl group of the primer suggests a novel, general approach for the design of antiviral compounds mimicking natural nucleosides and nucleotides.
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'''Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA'''
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Structural insights into mechanisms of catalysis and inhibition in Norwalk virus polymerase.,Zamyatkin DF, Parra F, Alonso JM, Harki DA, Peterson BR, Grochulski P, Ng KK J Biol Chem. 2008 Mar 21;283(12):7705-12. Epub 2008 Jan 9. PMID:18184655<ref>PMID:18184655</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3BSN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSN OCA].
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<div class="pdbe-citations 3bsn" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Norwalk virus]]
[[Category: Norwalk virus]]
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[[Category: Protein complex]]
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[[Category: Ng KKS]]
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[[Category: RNA-directed RNA polymerase]]
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[[Category: Zamyatkin DF]]
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[[Category: Ng, K K.S.]]
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[[Category: Zamyatkin, D F.]]
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[[Category: GOL]]
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[[Category: MN]]
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[[Category: N5C]]
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[[Category: antiviral enzyme inhibitor]]
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[[Category: helicase]]
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[[Category: hydrolase]]
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[[Category: nucleotide-binding]]
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[[Category: nucleotidyltransferase]]
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[[Category: rna replication]]
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[[Category: rna-dependent rna polymerase]]
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[[Category: rna-directed rna polymerase]]
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[[Category: transferase]]
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[[Category: transferase/rna complex]]
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[[Category: viral replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:00:59 2008''
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Current revision

Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA

PDB ID 3bsn

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