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5bvz
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==HK620 Tail Needle crystallized at pH 9 (Crystal form II)== | |
| + | <StructureSection load='5bvz' size='340' side='right'caption='[[5bvz]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5bvz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_HK620 Enterobacteria phage HK620]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4foh 4foh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BVZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bvz OCA], [https://pdbe.org/5bvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bvz RCSB], [https://www.ebi.ac.uk/pdbsum/5bvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bvz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9AYZ3_BPHK6 Q9AYZ3_BPHK6] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial viruses of the P22-like family encode a specialized tail needle essential for genome stabilization after DNA-packaging and implicated in Gram-negative cell envelope penetration. The atomic structure of P22 tail needle (gp26) crystallized at acidic pH reveals a slender fiber containing an N-terminal trimer-of-hairpins tip. Though the length and composition of tail needles vary significantly in Podoviridae, unexpectedly, the amino acid sequence of the N-terminal tip is exceptionally conserved in more than two hundred genomes of P22-like phages and prophages. In this paper, we used X-ray crystallography and EM to investigate the neutral pH structure of three tail needles from bacteriophage P22, HK620 and Sf6. In all cases, we found the N-terminal tip is poorly structured, in stark contrast to the compact trimer-of-hairpins seen in gp26 crystallized at acidic pH. Hydrogen/deuterium exchange mass spectrometry, limited proteolysis, circular dichroism spectroscopy and gel filtration chromatography revealed that the N-terminal tip is highly dynamic in solution and unlikely to adopt a stable trimeric conformation at physiological pH. This is supported by the cryo-EM reconstruction of P22 mature virion tail, where the density of gp26 N-terminal tip is incompatible with a trimer-of-hairpins. We propose the tail needle N-terminal tip exists in two conformations: a pre-ejection extended conformation, which seals the portal vertex after genome-packaging and a post-ejection trimer-of-hairpins that form upon its release from the virion. The conformational plasticity of the tail needle N-terminal tip is built in the amino acid sequence, explaining its extraordinary conservation in nature. | ||
| - | + | Structural plasticity of the protein plug that traps newly packaged genomes in podoviridae virions.,Bhardwaj A, Sankhala RS, Olia AS, Brooke D, Casjens SR, Taylor DJ, Prevelige PE Jr, Cingolani G J Biol Chem. 2015 Nov 16. pii: jbc.M115.696260. PMID:26574546<ref>PMID:26574546</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Bhardwaj | + | <div class="pdbe-citations 5bvz" style="background-color:#fffaf0;"></div> |
| - | [[Category: Cingolani | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Enterobacteria phage HK620]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bhardwaj A]] | ||
| + | [[Category: Cingolani G]] | ||
Current revision
HK620 Tail Needle crystallized at pH 9 (Crystal form II)
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