2n19
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==STIL binding to the Polo-box domain 3 of PLK4 regulates centriole duplication== | |
| + | <StructureSection load='2n19' size='340' side='right'caption='[[2n19]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2n19]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N19 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n19 OCA], [https://pdbe.org/2n19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n19 RCSB], [https://www.ebi.ac.uk/pdbsum/2n19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n19 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PLK4_HUMAN PLK4_HUMAN] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.<ref>PMID:16326102</ref> <ref>PMID:16244668</ref> <ref>PMID:17681131</ref> <ref>PMID:18239451</ref> <ref>PMID:19164942</ref> <ref>PMID:21725316</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Polo-like kinases (PLK) are eukaryotic regulators of cell cycle progression, mitosis and cytokinesis; PLK4 is a master regulator of centriole duplication. Here, we demonstrate that the SCL/TAL1 interrupting locus (STIL) protein interacts via its coiled-coil region (STIL-CC) with PLK4 in vivo. STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region. Structure determination of free PLK4-PB3 and its STIL-CC complex via NMR and crystallography reveals a novel mode of Polo-box-peptide interaction mimicking coiled-coil formation. In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding. We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication. | ||
| - | + | STIL binding to Polo-box 3 of PLK4 regulates centriole duplication.,Arquint C, Gabryjonczyk AM, Imseng S, Bohm R, Sauer E, Hiller S, Nigg EA, Maier T Elife. 2015 Jul 18;4. doi: 10.7554/eLife.07888. PMID:26188084<ref>PMID:26188084</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 2n19" style="background-color:#fffaf0;"></div> |
| - | [[Category: Boehm | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Hiller | + | __TOC__ |
| - | [[Category: Imseng | + | </StructureSection> |
| - | [[Category: | + | [[Category: Homo sapiens]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Arquint C]] |
| + | [[Category: Boehm R]] | ||
| + | [[Category: Gabryjonczyk A]] | ||
| + | [[Category: Hiller S]] | ||
| + | [[Category: Imseng S]] | ||
| + | [[Category: Maier T]] | ||
| + | [[Category: Nigg E]] | ||
| + | [[Category: Sauer E]] | ||
Current revision
STIL binding to the Polo-box domain 3 of PLK4 regulates centriole duplication
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Categories: Homo sapiens | Large Structures | Arquint C | Boehm R | Gabryjonczyk A | Hiller S | Imseng S | Maier T | Nigg E | Sauer E
