5avd

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'''Unreleased structure'''
 
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The entry 5avd is ON HOLD
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==The 0.86 angstrom structure of elastase crystallized in high-strength agarose hydrogel==
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<StructureSection load='5avd' size='340' side='right'caption='[[5avd]], [[Resolution|resolution]] 0.86&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5avd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AVD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AVD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5avd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5avd OCA], [https://pdbe.org/5avd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5avd RCSB], [https://www.ebi.ac.uk/pdbsum/5avd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5avd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG] Acts upon elastin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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High-throughput protein X-ray crystallography offers a significant opportunity to facilitate drug discovery. The most reliable approach is to determine the three-dimensional structure of the protein-ligand complex by soaking the ligand in apo crystals. However, protein apo crystals produced by conventional crystallization in a solution are fatally damaged by osmotic shock during soaking. To overcome this difficulty, we present a novel technique for growing protein crystals in a high-concentration hydrogel that is completely gellified and exhibits high strength. This technique allowed us essentially to increase the mechanical stability of the crystals, preventing serious damage to the crystals caused by osmotic shock. Thus, this method may accelerate structure-based drug discoveries.
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Authors: Sugiyama, S., Shimizu, N., Maruyama, M., Sazaki, G., Adachi, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Matsumura, H.
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Growth of protein crystals in hydrogels prevents osmotic shock.,Sugiyama S, Maruyama M, Sazaki G, Hirose M, Adachi H, Takano K, Murakami S, Inoue T, Mori Y, Matsumura H J Am Chem Soc. 2012 Apr 4;134(13):5786-9. doi: 10.1021/ja301584y. Epub 2012 Mar, 27. PMID:22435400<ref>PMID:22435400</ref>
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Description: The 0.86 angstrom structure of elastase crystallized in high-strength agarose hydrogel
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Inoue, T]]
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<div class="pdbe-citations 5avd" style="background-color:#fffaf0;"></div>
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[[Category: Takano, K]]
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[[Category: Mori, Y]]
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==See Also==
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[[Category: Matsumura, H]]
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*[[Elastase 3D structures|Elastase 3D structures]]
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[[Category: Shimizu, N]]
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== References ==
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[[Category: Murakami, S]]
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<references/>
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[[Category: Sugiyama, S]]
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__TOC__
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[[Category: Sazaki, G]]
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</StructureSection>
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[[Category: Maruyama, M]]
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[[Category: Large Structures]]
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[[Category: Adachi, H]]
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[[Category: Sus scrofa]]
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[[Category: Adachi H]]
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[[Category: Inoue T]]
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[[Category: Maruyama M]]
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[[Category: Matsumura H]]
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[[Category: Mori Y]]
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[[Category: Murakami S]]
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[[Category: Sazaki G]]
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[[Category: Shimizu N]]
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[[Category: Sugiyama S]]
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[[Category: Takano K]]

Current revision

The 0.86 angstrom structure of elastase crystallized in high-strength agarose hydrogel

PDB ID 5avd

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