5a31

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(New page: '''Unreleased structure''' The entry 5a31 is ON HOLD until sometime in the future Authors: Chang, L., Zhang, Z., Yang, J., Mclaughlin, S.H., Barford, D. Description: Structure of the h...)
Current revision (11:35, 9 May 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5a31 is ON HOLD until sometime in the future
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==Structure of the human APC-Cdh1-Hsl1-UbcH10 complex.==
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<SX load='5a31' size='340' side='right' viewer='molstar' caption='[[5a31]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5a31]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A31 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a31 OCA], [https://pdbe.org/5a31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a31 RCSB], [https://www.ebi.ac.uk/pdbsum/5a31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a31 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/APC1_HUMAN APC1_HUMAN] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The anaphase-promoting complex (APC/C) is a multimeric RING E3 ubiquitin ligase that controls chromosome segregation and mitotic exit. Its regulation by coactivator subunits, phosphorylation, the mitotic checkpoint complex and interphase early mitotic inhibitor 1 (Emi1) ensures the correct order and timing of distinct cell-cycle transitions. Here we use cryo-electron microscopy to determine atomic structures of APC/C-coactivator complexes with either Emi1 or a UbcH10-ubiquitin conjugate. These structures define the architecture of all APC/C subunits, the position of the catalytic module and explain how Emi1 mediates inhibition of the two E2s UbcH10 and Ube2S. Definition of Cdh1 interactions with the APC/C indicates how they are antagonized by Cdh1 phosphorylation. The structure of the APC/C with UbcH10-ubiquitin reveals insights into the initiating ubiquitination reaction. Our results provide a quantitative framework for the design of future experiments to investigate APC/C functions in vivo.
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Authors: Chang, L., Zhang, Z., Yang, J., Mclaughlin, S.H., Barford, D.
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Atomic structure of the APC/C and its mechanism of protein ubiquitination.,Chang L, Zhang Z, Yang J, McLaughlin SH, Barford D Nature. 2015 Jun 25;522(7557):450-4. doi: 10.1038/nature14471. Epub 2015 Jun 15. PMID:26083744<ref>PMID:26083744</ref>
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Description: Structure of the human APC-Cdh1-Hsl1-UbcH10 complex.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Mclaughlin, S.H]]
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<div class="pdbe-citations 5a31" style="background-color:#fffaf0;"></div>
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[[Category: Zhang, Z]]
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== References ==
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[[Category: Chang, L]]
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<references/>
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[[Category: Yang, J]]
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__TOC__
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[[Category: Barford, D]]
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</SX>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Barford D]]
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[[Category: Chang L]]
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[[Category: Mclaughlin SH]]
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[[Category: Yang J]]
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[[Category: Zhang Z]]

Current revision

Structure of the human APC-Cdh1-Hsl1-UbcH10 complex.

5a31, resolution 4.30Å

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