5avn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "5avn" [edit=sysop:move=sysop])
Current revision (15:49, 8 November 2023) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5avn is ON HOLD
+
==The 1.03 angstrom structure (P212121) of glucose isomerase crystallized in high-strength agarose hydrogel==
 +
<StructureSection load='5avn' size='340' side='right'caption='[[5avn]], [[Resolution|resolution]] 1.03&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5avn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AVN FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.03&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5avn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5avn OCA], [https://pdbe.org/5avn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5avn RCSB], [https://www.ebi.ac.uk/pdbsum/5avn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5avn ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU] Involved in D-xylose catabolism.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
High-throughput protein X-ray crystallography offers a significant opportunity to facilitate drug discovery. The most reliable approach is to determine the three-dimensional structure of the protein-ligand complex by soaking the ligand in apo crystals. However, protein apo crystals produced by conventional crystallization in a solution are fatally damaged by osmotic shock during soaking. To overcome this difficulty, we present a novel technique for growing protein crystals in a high-concentration hydrogel that is completely gellified and exhibits high strength. This technique allowed us essentially to increase the mechanical stability of the crystals, preventing serious damage to the crystals caused by osmotic shock. Thus, this method may accelerate structure-based drug discoveries.
-
Authors: Sugiyama, S., Shimizu, N., Maruyama, N., Sazaki, G., Adachi, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Matsumura, H.
+
Growth of protein crystals in hydrogels prevents osmotic shock.,Sugiyama S, Maruyama M, Sazaki G, Hirose M, Adachi H, Takano K, Murakami S, Inoue T, Mori Y, Matsumura H J Am Chem Soc. 2012 Apr 4;134(13):5786-9. doi: 10.1021/ja301584y. Epub 2012 Mar, 27. PMID:22435400<ref>PMID:22435400</ref>
-
Description: The 1.03 angstrom structure (P212121) of glucose isomerase crystallized in high-strength agarose hydrogel
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Inoue, T]]
+
<div class="pdbe-citations 5avn" style="background-color:#fffaf0;"></div>
-
[[Category: Maruyama, N]]
+
== References ==
-
[[Category: Takano, K]]
+
<references/>
-
[[Category: Mori, Y]]
+
__TOC__
-
[[Category: Matsumura, H]]
+
</StructureSection>
-
[[Category: Shimizu, N]]
+
[[Category: Large Structures]]
-
[[Category: Murakami, S]]
+
[[Category: Streptomyces rubiginosus]]
-
[[Category: Sugiyama, S]]
+
[[Category: Adachi H]]
-
[[Category: Sazaki, G]]
+
[[Category: Inoue T]]
-
[[Category: Adachi, H]]
+
[[Category: Maruyama N]]
 +
[[Category: Matsumura H]]
 +
[[Category: Mori Y]]
 +
[[Category: Murakami S]]
 +
[[Category: Sazaki G]]
 +
[[Category: Shimizu N]]
 +
[[Category: Sugiyama S]]
 +
[[Category: Takano K]]

Current revision

The 1.03 angstrom structure (P212121) of glucose isomerase crystallized in high-strength agarose hydrogel

PDB ID 5avn

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5avn"
Personal tools