5c6k
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Bacteriophage P2 integrase catalytic domain== | |
| + | <StructureSection load='5c6k' size='340' side='right'caption='[[5c6k]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5c6k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P2 Escherichia virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C6K FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6k OCA], [https://pdbe.org/5c6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c6k RCSB], [https://www.ebi.ac.uk/pdbsum/5c6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/VINT_BPP2 VINT_BPP2] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteriophage P2 is a temperate phage capable of integrating its DNA into the host genome by site-specific recombination upon lysogenization. Integration and excision of the phage genome requires P2 integrase, which performs recognition, cleavage and joining of DNA during these processes. This work presents the high-resolution crystal structure of the catalytic domain of P2 integrase, and analysis of the structure-function relationship of several previously identified non-functional P2 integrase mutants. The DNA binding area is characterized by a large positively charged patch, harboring key residues. The structure reveals potential for large dimer flexibility, likely essential for rearrangement of DNA strands upon integration and excision of the phage DNA. | ||
| - | + | Crystal structure of the bacteriophage P2 integrase catalytic domain.,Skaar K, Claesson M, Odegrip R, Hogbom M, Haggard-Ljungquist E, Stenmark P FEBS Lett. 2015 Oct 8. pii: S0014-5793(15)00875-3. doi:, 10.1016/j.febslet.2015.09.026. PMID:26453836<ref>PMID:26453836</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5c6k" style="background-color:#fffaf0;"></div> |
| - | [[Category: Eriksson | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]] |
| - | [[Category: Odegrip | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Escherichia virus P2]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Claesson M]] | ||
| + | [[Category: Eriksson J]] | ||
| + | [[Category: Haggard-Ljungquist E]] | ||
| + | [[Category: Hogbom M]] | ||
| + | [[Category: Odegrip R]] | ||
| + | [[Category: Skaar K]] | ||
| + | [[Category: Stenmark P]] | ||
Current revision
Bacteriophage P2 integrase catalytic domain
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