4xyp

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==Crystal structure of a piscine viral fusion protein==
==Crystal structure of a piscine viral fusion protein==
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<StructureSection load='4xyp' size='340' side='right' caption='[[4xyp]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='4xyp' size='340' side='right'caption='[[4xyp]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4xyp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XYP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XYP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4xyp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmon_isavirus Salmon isavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XYP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MBT:3,7-BIS(DIMETHYLAMINO)PHENOTHIAZIN-5-IUM'>MBT</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xyp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xyp RCSB], [http://www.ebi.ac.uk/pdbsum/4xyp PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MBT:3,7-BIS(DIMETHYLAMINO)PHENOTHIAZIN-5-IUM'>MBT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xyp OCA], [https://pdbe.org/4xyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xyp RCSB], [https://www.ebi.ac.uk/pdbsum/4xyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xyp ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/C6F339_9ORTO C6F339_9ORTO]
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Segment 5, open reading frame (ORF) 1 of the infectious salmon anemia virus (ISAV) genome encodes for the ISAV F protein, which is responsible for viral-host endosomal membrane fusion during a productive ISAV infection. The entry machinery of ISAV is comprised of a complex of the ISAV F and ISAV HE proteins in an unknown stoichiometry prior to receptor engagement by ISAV HE. Following binding of the receptor to ISAV HE, dissociation of the ISAV F protein from HE and subsequent endocytosis, the ISAV F protein resolves into a fusion competent oligomeric state. Here, we present a 2.1 A crystal structure of the fusion core of the ISAV F protein solved at low pH. This structure has allowed us to unambiguously demonstrate that the ISAV entry machinery exhibits typical Class I viral fusion protein architecture. Furthermore, we have determined stabilizing factors that accommodate the pH-dependent mode of ISAV transmission and our structure has allowed the identification of a central coil that is conserved across numerous and varied post-fusion viral glycoprotein structures. We then discuss a mechanistic model of ISAV fusion that parallels the paramyxoviral Class I fusion strategy wherein attachment and fusion are relegated to separate proteins in a similar fashion to ISAV fusion.
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Electrostatic Architecture of the Infectious Salmon Anemia Virus (ISAV) Core Fusion Protein Illustrates a Carboxyl-Carboxylate pH-Sensor.,Cook JD, Soto-Montoya H, Korpela MK, Lee JE J Biol Chem. 2015 Jun 16. pii: jbc.M115.644781. PMID:26082488<ref>PMID:26082488</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cook, J D]]
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[[Category: Large Structures]]
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[[Category: Lee, J E]]
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[[Category: Salmon isavirus]]
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[[Category: 6-helix bundle]]
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[[Category: Cook JD]]
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[[Category: Class i viral fusion protein]]
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[[Category: Lee JE]]
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[[Category: Heptad repeat]]
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[[Category: Viral protein]]
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Current revision

Crystal structure of a piscine viral fusion protein

PDB ID 4xyp

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