4xgy

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==GFP based antibody (fluorobody)==
==GFP based antibody (fluorobody)==
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<StructureSection load='4xgy' size='340' side='right' caption='[[4xgy]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
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<StructureSection load='4xgy' size='340' side='right'caption='[[4xgy]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4xgy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XGY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4xgy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria], [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XGY FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.494&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2b3p|2b3p]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgy OCA], [https://pdbe.org/4xgy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xgy RCSB], [https://www.ebi.ac.uk/pdbsum/4xgy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xgy ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xgy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xgy RCSB], [http://www.ebi.ac.uk/pdbsum/4xgy PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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[https://www.uniprot.org/uniprot/A0A059PIQ0_AEQVI A0A059PIQ0_AEQVI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacterium Vibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space group P43212, with unit-cell parameters a = b = 63.35, c = 125.90 A, were obtained by vapour diffusion in hanging drops and the structure was refined to an Rfree of 16.7% at 1.5 A resolution. The structure shows a CDR loop of the antibody on the GFP scaffold.
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The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus.,Chen Y, Huang X, Wang R, Wang S, Shi N Acta Crystallogr F Struct Biol Commun. 2015 Jul;71(Pt 7):913-8. doi:, 10.1107/S2053230X15008845. Epub 2015 Jun 27. PMID:26144238<ref>PMID:26144238</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4xgy" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Chen, Y G]]
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[[Category: Aequorea victoria]]
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[[Category: Shi, N]]
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[[Category: Large Structures]]
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[[Category: Wang, S H]]
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[[Category: Synthetic construct]]
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[[Category: Cdr3]]
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[[Category: Vibrio parahaemolyticus]]
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[[Category: Fluorescent protein]]
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[[Category: Chen YG]]
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[[Category: Fluorobody]]
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[[Category: Shi N]]
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[[Category: Gfp]]
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[[Category: Wang SH]]

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GFP based antibody (fluorobody)

PDB ID 4xgy

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