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4ygw

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==RNase S in complex with stabilized S peptide==
==RNase S in complex with stabilized S peptide==
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<StructureSection load='4ygw' size='340' side='right' caption='[[4ygw]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
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<StructureSection load='4ygw' size='340' side='right'caption='[[4ygw]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ygw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YGW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ygw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YGW FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4Y8:1-HYDROXYPROPAN-2-ONE'>4Y8</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4Y8:1-HYDROXYPROPAN-2-ONE'>4Y8</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ygw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ygw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ygw RCSB], [http://www.ebi.ac.uk/pdbsum/4ygw PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ygw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ygw OCA], [https://pdbe.org/4ygw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ygw RCSB], [https://www.ebi.ac.uk/pdbsum/4ygw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ygw ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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Macrocyclization is a broadly applied approach for overcoming the intrinsically disordered nature of linear peptides. Herein, it is shown that dichloroacetone (DCA) enhances helical secondary structures when introduced between peptide nucleophiles, such as thiols, to yield an acetone-linked bridge (ACE). Aside from stabilizing helical structures, the ketone moiety embedded in the linker can be modified with diverse molecular tags by oxime ligation. Insights into the structure of the tether were obtained through co-crystallization of a constrained S-peptide in complex with RNAse S. The scope of the acetone-linked peptides was further explored through the generation of N-terminus to side chain macrocycles and a new approach for generating fused macrocycles (bicycles). Together, these studies suggest that acetone linking is generally applicable to peptide macrocycles with a specific utility in the synthesis of stabilized helices that incorporate functional tags.
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Acetone-Linked Peptides: A Convergent Approach for Peptide Macrocyclization and Labeling.,Assem N, Ferreira DJ, Wolan DW, Dawson PE Angew Chem Int Ed Engl. 2015 Jun 11. doi: 10.1002/anie.201502607. PMID:26096515<ref>PMID:26096515</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Assem, N]]
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[[Category: Bos taurus]]
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[[Category: Dawson, P E]]
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[[Category: Large Structures]]
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[[Category: Ferreira, D]]
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[[Category: Synthetic construct]]
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[[Category: Wolan, D W]]
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[[Category: Assem N]]
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[[Category: Hydrolase]]
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[[Category: Dawson PE]]
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[[Category: Rnase]]
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[[Category: Ferreira D]]
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[[Category: Side chain link]]
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[[Category: Wolan DW]]
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[[Category: Unnatural amino acid]]
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Current revision

RNase S in complex with stabilized S peptide

PDB ID 4ygw

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