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| - | [[Image:422d.gif|left|200px]] | |
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| - | {{Structure
| + | ==5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'== |
| - | |PDB= 422d |SIZE=350|CAPTION= <scene name='initialview01'>422d</scene>, resolution 2.60Å
| + | <StructureSection load='422d' size='340' side='right'caption='[[422d]], [[Resolution|resolution]] 2.60Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[422d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=422D FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=422d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=422d OCA], [https://pdbe.org/422d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=422d RCSB], [https://www.ebi.ac.uk/pdbsum/422d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=422d ProSAT]</span></td></tr> |
| - | }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3''''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Eswaramoorthy S]] |
| - | ==Overview== | + | [[Category: Rao ST]] |
| - | The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.
| + | [[Category: Sundaralingam M]] |
| - | | + | |
| - | ==About this Structure== | + | |
| - | 422D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA].
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| - | ==Reference==
| + | |
| - | Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides., Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684886 14684886]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Eswaramoorthy, S.]] | + | |
| - | [[Category: Rao, S T.]] | + | |
| - | [[Category: Sundaralingam, M.]] | + | |
| - | [[Category: double helix]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:06 2008''
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