Antigen 85
From Proteopedia
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| - | <StructureSection load=' | + | <StructureSection load='' size='350' side='right' caption='Antigen 85C complex with detergent octylthioglucoside (PDB code [[1va5]])' scene='70/706736/Cv/1'> |
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== Function == | == Function == | ||
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| + | '''Antigens 85A, 85B and 85C''' posses mycolyltransferase activity and generate trehalose dimycolate which is an essential envelope lipid for ''Mycobacterium tuberculosis'' virulence. Inhibition of these enzymes hinders growth of the bacteria. The three enzymes show very high sequence identity (68-79%). | ||
== Disease == | == Disease == | ||
| - | + | Antigen 85 proteins levels are elevated in tuberculosis patients. | |
== Structural highlights == | == Structural highlights == | ||
| - | </ | + | The 3D structures of the 3 antigens show that their active sites are virtually identical indicating that they share the same substrate. However, residues at the surface of the enzymes are different. |
| + | *<scene name='70/706736/Cv/5'>Click here to see active site</scene>.<ref>PMID:15192106</ref> | ||
== 3D Structures of antigen 85 == | == 3D Structures of antigen 85 == | ||
| + | [[Antigen 85 3D structures]] | ||
| - | + | </StructureSection> | |
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| - | **[[1dqz]] – Mt85C <br /> | ||
| - | **[[4mql]], [[4qdx]], [[4qdz]], [[4qe3]], [[4qek]] – Mt85C (mutant)<br /> | ||
| - | **[[1dqy]] – Mt85C + diethyl phosphate inhibitor <br /> | ||
| - | **[[4mqm]], [[4qdu]] – Mt85C + ebselen inhibitor <br /> | ||
| - | **[[4qdo]] – Mt85C + chloromercurybenzoate inhibitor <br /> | ||
| - | **[[4qdt]] – Mt85C + iodoacetamide inhibitor <br /> | ||
| - | **[[1va5]] – Mt85C + octyl glucoside <br /> | ||
| - | **[[3hrh]] – Mt85C + glycerol <br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC. Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity. J Biol Chem. 2004 Aug 27;279(35):36771-7. Epub 2004 Jun 10. PMID:15192106 doi:http://dx.doi.org/10.1074/jbc.M400811200
