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| - | [[Image:5ana.gif|left|200px]] | |
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| - | {{Structure
| + | ==THE CRYSTAL STRUCTURE OF D(GTACGTAC) AT 2.25 ANGSTROMS RESOLUTION. ARE THE A-DNA'S ALWAYS UNWOUND APPROXIMATELY 10 DEGREES AT THE C-G STEPS== |
| - | |PDB= 5ana |SIZE=350|CAPTION= <scene name='initialview01'>5ana</scene>, resolution 2.250Å
| + | <StructureSection load='5ana' size='340' side='right'caption='[[5ana]], [[Resolution|resolution]] 2.25Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
| + | <table><tr><td colspan='2'>[[5ana]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ANA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ANA FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ana FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ana OCA], [https://pdbe.org/5ana PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ana RCSB], [https://www.ebi.ac.uk/pdbsum/5ana PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ana ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''THE CRYSTAL STRUCTURE OF D(GTACGTAC) AT 2.25 ANGSTROMS RESOLUTION. ARE THE A-DNA'S ALWAYS UNWOUND APPROXIMATELY 10 DEGREES AT THE C-G STEPS'''
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | ==Overview== | + | [[Category: Takusagawa F]] |
| - | The structure of the self-complementary octamer d(GTACGTAC) has been analyzed by a single crystal X-ray diffraction method at 2.25 A resolution. The crystallographic R factor was 0.184 for all 1233 reflections at this resolution. In spite of the alternating purine-pyrimidine sequence, d(GTACGTAC) adopts the A-form conformation rather than the left-handed Z-form. The average helix twist and the mean rise per base pair are 32.1 degrees and 3.18 A, respectively. The d(GTACGTAC) helix is characterized by a wide open major groove and small base-pair tilt (9.7 degrees). The partial unwinding of the helix is observed only at the central pyrimidine-purine C-G step, but not at the other pyrimidine-purine T-A steps. Based on this study and six other X-ray studies, we propose a hypothesis that the A-DNA's are always unwound approximately 10 degrees at the C-G steps. Significant differences in base-pair stacking modes are seen between the purine-pyrimidine step and the pyrimidine-purine step. All deoxyribose rings adopt the C3'-endo conformation. All backbone torsion angles fall into the range expected for the A-DNA form, except for the nucleotide G5, whose alpha and gamma torsion angles adopt the trans, trans conformation instead of the common gauche-, gauche+ conformation.
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| - | | + | |
| - | ==About this Structure== | + | |
| - | 5ANA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ANA OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | The crystal structure of d(GTACGTAC) at 2.25 A resolution: are the A-DNA's always unwound approximately 10 degrees at the C-G steps?, Takusagawa F, J Biomol Struct Dyn. 1990 Feb;7(4):795-809. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2310515 2310515]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Takusagawa, F.]]
| + | |
| - | [[Category: MG]] | + | |
| - | [[Category: a-dna]] | + | |
| - | [[Category: double helix]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:11:31 2008'' | + | |
