5cf9

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==Cleavage of nicotinamide adenine dinucleotide by the ribosome inactivating protein of Momordica charantia - enzyme-NADP+ co-crystallisation.==
==Cleavage of nicotinamide adenine dinucleotide by the ribosome inactivating protein of Momordica charantia - enzyme-NADP+ co-crystallisation.==
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<StructureSection load='5cf9' size='340' side='right' caption='[[5cf9]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
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<StructureSection load='5cf9' size='340' side='right'caption='[[5cf9]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5cf9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CF9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CF9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5cf9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CF9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CF9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.52&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ypt|4ypt]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cf9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cf9 OCA], [https://pdbe.org/5cf9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cf9 RCSB], [https://www.ebi.ac.uk/pdbsum/5cf9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cf9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cf9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cf9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5cf9 RCSB], [http://www.ebi.ac.uk/pdbsum/5cf9 PDBsum]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIP1_MOMCH RIP1_MOMCH]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The interaction of momordin, a type 1 ribosome-inactivating protein from Momordica charantia, with NADP(+) and NADPH has been investigated by X-ray diffraction analysis of complexes generated by co-crystallization and crystal soaking. It is known that the proteins of this family readily cleave the adenine-ribose bond of adenosine and related nucleotides in the crystal, leaving the product, adenine, bound to the enzyme active site. Surprisingly, the nicotinamide-ribose bond of oxidized NADP(+) is cleaved, leaving nicotinamide bound in the active site in the same position but in a slightly different orientation to that of the five-membered ring of adenine. No binding or cleavage of NADPH was observed at pH 7.4 in these experiments. These observations are in accord with current views of the enzyme mechanism and may contribute to ongoing searches for effective inhibitors.
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Cleavage of nicotinamide adenine dinucleotide by the ribosome-inactivating protein from Momordica charantia.,Vinkovic M, Dunn G, Wood GE, Husain J, Wood SP, Gill R Acta Crystallogr F Struct Biol Commun. 2015 Sep 1;71(Pt 9):1152-5. doi:, 10.1107/S2053230X15013540. Epub 2015 Aug 25. PMID:26323301<ref>PMID:26323301</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5cf9" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Momordica charantia]]
[[Category: Momordica charantia]]
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[[Category: RRNA N-glycosylase]]
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[[Category: Dunn G]]
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[[Category: Dunn, G]]
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[[Category: Gill R]]
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[[Category: Gill, R]]
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[[Category: Husain J]]
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[[Category: Husain, J]]
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[[Category: Vinkovic M]]
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[[Category: Vinkovic, M]]
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[[Category: Wood GE]]
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[[Category: Wood, G E]]
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[[Category: Wood SP]]
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[[Category: Wood, S P]]
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[[Category: Complex]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Nadp]]
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[[Category: Product]]
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Current revision

Cleavage of nicotinamide adenine dinucleotide by the ribosome inactivating protein of Momordica charantia - enzyme-NADP+ co-crystallisation.

PDB ID 5cf9

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