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| | ==Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.3-H4 dimer== | | ==Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.3-H4 dimer== |
| - | <StructureSection load='5bnx' size='340' side='right' caption='[[5bnx]], [[Resolution|resolution]] 2.31Å' scene=''> | + | <StructureSection load='5bnx' size='340' side='right'caption='[[5bnx]], [[Resolution|resolution]] 2.31Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5bnx]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BNX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5bnx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BNX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.305Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5bnv|5bnv]], [[5bo0|5bo0]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bnx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5bnx RCSB], [http://www.ebi.ac.uk/pdbsum/5bnx PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bnx OCA], [https://pdbe.org/5bnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bnx RCSB], [https://www.ebi.ac.uk/pdbsum/5bnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bnx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ASF1B_HUMAN ASF1B_HUMAN]] Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.<ref>PMID:11897662</ref> <ref>PMID:12842904</ref> <ref>PMID:14718166</ref> <ref>PMID:15664198</ref> <ref>PMID:16151251</ref> [[http://www.uniprot.org/uniprot/MCM2_HUMAN MCM2_HUMAN]] Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.<ref>PMID:8175912</ref> | + | [https://www.uniprot.org/uniprot/H33_HUMAN H33_HUMAN] |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | During DNA replication, chromatin is reassembled by recycling of modified old histones and deposition of new ones. How histone dynamics integrates with DNA replication to maintain genome and epigenome information remains unclear. Here, we reveal how human MCM2, part of the replicative helicase, chaperones histones H3-H4. Our first structure shows an H3-H4 tetramer bound by two MCM2 histone-binding domains (HBDs), which hijack interaction sites used by nucleosomal DNA. Our second structure reveals MCM2 and ASF1 cochaperoning an H3-H4 dimer. Mutational analyses show that the MCM2 HBD is required for MCM2-7 histone-chaperone function and normal cell proliferation. Further, we show that MCM2 can chaperone both new and old canonical histones H3-H4 as well as H3.3 and CENPA variants. The unique histone-binding mode of MCM2 thus endows the replicative helicase with ideal properties for recycling histones genome wide during DNA replication.
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| - | A unique binding mode enables MCM2 to chaperone histones H3-H4 at replication forks.,Huang H, Stromme CB, Saredi G, Hodl M, Strandsby A, Gonzalez-Aguilera C, Chen S, Groth A, Patel DJ Nat Struct Mol Biol. 2015 Jul 13. doi: 10.1038/nsmb.3055. PMID:26167883<ref>PMID:26167883</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | ==See Also== |
| - | </div>
| + | *[[Anti-silencing factor 3D structures|Anti-silencing factor 3D structures]] |
| - | == References == | + | *[[Histone 3D structures|Histone 3D structures]] |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Huang, H]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Patel, D J]] | + | [[Category: Large Structures]] |
| - | [[Category: Asf1]] | + | [[Category: Huang H]] |
| - | [[Category: Chaperone-dna binding protein complex]] | + | [[Category: Patel DJ]] |
| - | [[Category: Dna replication]]
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| - | [[Category: H3 3-h4 dimer]]
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| - | [[Category: Mcm2]]
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