2n5i
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==PltL-pyrrolyl== | |
| + | <StructureSection load='2n5i' size='340' side='right'caption='[[2n5i]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2n5i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_protegens_Pf-5 Pseudomonas protegens Pf-5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N5I FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4EM:(3R)-3-HYDROXY-2,2-DIMETHYL-4-OXO-4-{[3-OXO-3-({2-[(1H-PYRROL-2-YLCARBONYL)AMINO]ETHYL}AMINO)PROPYL]AMINO}BUTYL+DIHYDROGEN+PHOSPHATE'>4EM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n5i OCA], [https://pdbe.org/2n5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n5i RCSB], [https://www.ebi.ac.uk/pdbsum/2n5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n5i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type II nonribosomal peptide synthetases (NRPS) generate exotic amino acid derivatives that, combined with additional pathways, form many bioactive natural products. One family of type II NRPSs produce pyrrole moieties, which commonly arise from proline oxidation while tethered to a conserved, type II peptidyl carrier protein (PCP), as exemplified by PltL in the biosynthesis of pyoluteorin. We sought to understand the structural role of pyrrole PCPs in substrate and protein interactions through the study of pyrrole analogs tethered to PltL. Solution-phase NMR structural analysis revealed key interactions in residues of helix II and III with a bound pyrrole moiety. Conservation of these residues among PCPs in other pyrrole containing pathways suggests a conserved mechanism for formation, modification, and incorporation of pyrrole moieties. Further NOE analysis provided a unique pyrrole binding motif, offering accurate substrate positioning within the cleft between helices II and III. The overall structure resembles other PCPs but contains a unique conformation for helix III. This provides evidence of sequestration by the PCP of aromatic pyrrole substrates, illustrating the importance of substrate protection and regulation in type II NRPS systems. | ||
| - | + | Structure and Substrate Sequestration in the Pyoluteorin Type II Peptidyl Carrier Protein PltL.,Jaremko MJ, Lee DJ, Opella SJ, Burkart MD J Am Chem Soc. 2015 Sep 16;137(36):11546-9. doi: 10.1021/jacs.5b04525. Epub 2015 , Sep 4. PMID:26340431<ref>PMID:26340431</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Burkart | + | <div class="pdbe-citations 2n5i" style="background-color:#fffaf0;"></div> |
| - | [[Category: Jaremko | + | == References == |
| - | [[Category: Lee | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas protegens Pf-5]] | ||
| + | [[Category: Burkart MD]] | ||
| + | [[Category: Jaremko MJ]] | ||
| + | [[Category: Lee DJ]] | ||
Current revision
PltL-pyrrolyl
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