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| | ==Hha-H-NS46 charge zipper complex== | | ==Hha-H-NS46 charge zipper complex== |
| - | <StructureSection load='2mw2' size='340' side='right' caption='[[2mw2]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2mw2' size='340' side='right'caption='[[2mw2]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mw2]] is a 3 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MW2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mw2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MW2 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jw2|1jw2]], [[1ni8|1ni8]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mw2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mw2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mw2 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mw2 OCA], [https://pdbe.org/2mw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mw2 RCSB], [https://www.ebi.ac.uk/pdbsum/2mw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mw2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HHA_ECOLI HHA_ECOLI]] Down-regulates hemolysin production and can also stimulate transposition events in vivo. Binds DNA and influences DNA topology in response to environmental stimuli. Involved in persister cell formation, acting downstream of mRNA interferase (toxin) MqsR. Decreases biofilm formation by repressing the transcription of fimbrial genes fimA and ihfA, and by repressing the transcription of tRNAs corresponding to rare codons, which are abundant in type 1 fimbrial genes.<ref>PMID:1956303</ref> <ref>PMID:16317765</ref> <ref>PMID:18545668</ref> <ref>PMID:19909729</ref> [[http://www.uniprot.org/uniprot/HNS_ECOLI HNS_ECOLI]] A DNA-binding protein implicated in transcriptional repression (silencing) as well as in bacterial chromosome organization. H-NS binds tightly to AT-rich dsDNA, increases its thermal stability and inhibits transcription. Also binds to ssDNA and RNA but with a much lower affinity. H-NS has possible histone-like function. May be a global transcriptional regulator through its ability to bind to curved DNA sequences, which are found in regions upstream of a certain subset of promoters. Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD. Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its role is antagonized by LeuO. Subject to transcriptional auto-repression. Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150.<ref>PMID:7934818</ref> <ref>PMID:11031114</ref> <ref>PMID:17010156</ref> <ref>PMID:20659289</ref> | + | [https://www.uniprot.org/uniprot/HHA_ECOLI HHA_ECOLI] Down-regulates hemolysin production and can also stimulate transposition events in vivo. Binds DNA and influences DNA topology in response to environmental stimuli. Involved in persister cell formation, acting downstream of mRNA interferase (toxin) MqsR. Decreases biofilm formation by repressing the transcription of fimbrial genes fimA and ihfA, and by repressing the transcription of tRNAs corresponding to rare codons, which are abundant in type 1 fimbrial genes.<ref>PMID:1956303</ref> <ref>PMID:16317765</ref> <ref>PMID:18545668</ref> <ref>PMID:19909729</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bernado, P]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Cordeiro, T N]] | + | [[Category: Large Structures]] |
| - | [[Category: Garcia, J]] | + | [[Category: Bernado P]] |
| - | [[Category: Millet, O]] | + | [[Category: Cordeiro TN]] |
| - | [[Category: Pons, M]] | + | [[Category: Garcia J]] |
| - | [[Category: Charge-zipper complex]] | + | [[Category: Millet O]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Pons M]] |
| - | [[Category: Electrostatic-driven function]]
| + | |
| - | [[Category: Nucleoid-associated protein]]
| + | |
| - | [[Category: Salt-dependent dynamic]]
| + | |
| Structural highlights
Function
HHA_ECOLI Down-regulates hemolysin production and can also stimulate transposition events in vivo. Binds DNA and influences DNA topology in response to environmental stimuli. Involved in persister cell formation, acting downstream of mRNA interferase (toxin) MqsR. Decreases biofilm formation by repressing the transcription of fimbrial genes fimA and ihfA, and by repressing the transcription of tRNAs corresponding to rare codons, which are abundant in type 1 fimbrial genes.[1] [2] [3] [4]
References
- ↑ Nieto JM, Carmona M, Bolland S, Jubete Y, de la Cruz F, Juarez A. The hha gene modulates haemolysin expression in Escherichia coli. Mol Microbiol. 1991 May;5(5):1285-93. PMID:1956303
- ↑ Barrios AF, Zuo R, Ren D, Wood TK. Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility. Biotechnol Bioeng. 2006 Jan 5;93(1):188-200. PMID:16317765 doi:http://dx.doi.org/10.1002/bit.20681
- ↑ Garcia-Contreras R, Zhang XS, Kim Y, Wood TK. Protein translation and cell death: the role of rare tRNAs in biofilm formation and in activating dormant phage killer genes. PLoS One. 2008 Jun 11;3(6):e2394. doi: 10.1371/journal.pone.0002394. PMID:18545668 doi:http://dx.doi.org/10.1371/journal.pone.0002394
- ↑ Kim Y, Wood TK. Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli. Biochem Biophys Res Commun. 2010 Jan 1;391(1):209-13. doi:, 10.1016/j.bbrc.2009.11.033. Epub 2009 Nov 10. PMID:19909729 doi:10.1016/j.bbrc.2009.11.033
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