5a42
From Proteopedia
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==Cryo-EM single particle 3D reconstruction of the native conformation of E. coli alpha-2-macroglobulin (ECAM)== | ==Cryo-EM single particle 3D reconstruction of the native conformation of E. coli alpha-2-macroglobulin (ECAM)== | ||
| - | < | + | <SX load='5a42' size='340' side='right' viewer='molstar' caption='[[5a42]], [[Resolution|resolution]] 16.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5a42]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A42 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5a42]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A42 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a42 OCA], [https://pdbe.org/5a42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a42 RCSB], [https://www.ebi.ac.uk/pdbsum/5a42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a42 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A2MG_ECOLI A2MG_ECOLI] Protects the bacterial cell from host peptidases (PubMed:18697741, PubMed:26143919, PubMed:26100869). Acts by a 'trapping' mechanism. Cleavage of the bait-region domain by host peptidases leads to a global conformational change, which results in entrapment of the host peptidase and activation of the thioester bond that covalently binds the attacking host peptidase (PubMed:26143919, PubMed:26100869). Trapped peptidases are still active except against very large substrates (PubMed:26100869). May protect the entire periplam, including the lipoproteins anchored to the periplasmic side of the outer membrane, against intruding endopeptidases (PubMed:26100869).<ref>PMID:18697741</ref> <ref>PMID:26100869</ref> <ref>PMID:26143919</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The survival of commensal bacteria requires them to evade host peptidases. Gram-negative bacteria from the human gut microbiome encode a relative of the human endopeptidase inhibitor, alpha2-macroglobulin (alpha2M). Escherichia coli alpha2M (ECAM) is a approximately 180-kDa multidomain membrane-anchored pan-peptidase inhibitor, which is cleaved by host endopeptidases in an accessible bait region. Structural studies by electron microscopy and crystallography reveal that this cleavage causes major structural rearrangement of more than half the 13-domain structure from a native to a compact induced form. It also exposes a reactive thioester bond, which covalently traps the peptidase. Subsequently, peptidase-laden ECAM is shed from the membrane and may dimerize. Trapped peptidases are still active except against very large substrates, so inhibition potentially prevents damage of large cell envelope components, but not host digestion. Mechanistically, these results document a novel monomeric "snap trap." | ||
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| + | Structural and functional insights into Escherichia coli alpha2-macroglobulin endopeptidase snap-trap inhibition.,Garcia-Ferrer I, Arede P, Gomez-Blanco J, Luque D, Duquerroy S, Caston JR, Goulas T, Gomis-Ruth FX Proc Natl Acad Sci U S A. 2015 Jun 22. pii: 201506538. PMID:26100869<ref>PMID:26100869</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5a42" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
| - | [[Category: Arede | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Caston | + | [[Category: Large Structures]] |
| - | [[Category: Duquerroy | + | [[Category: Arede P]] |
| - | [[Category: Garcia-Ferrer | + | [[Category: Caston JR]] |
| - | [[Category: Gomez-Blanco | + | [[Category: Duquerroy S]] |
| - | [[Category: Gomis-Ruth | + | [[Category: Garcia-Ferrer I]] |
| - | [[Category: Goulas | + | [[Category: Gomez-Blanco J]] |
| - | [[Category: Luque | + | [[Category: Gomis-Ruth FX]] |
| - | + | [[Category: Goulas T]] | |
| - | + | [[Category: Luque D]] | |
Current revision
Cryo-EM single particle 3D reconstruction of the native conformation of E. coli alpha-2-macroglobulin (ECAM)
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