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4tqk

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==Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection==
==Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection==
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<StructureSection load='4tqk' size='340' side='right' caption='[[4tqk]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='4tqk' size='340' side='right'caption='[[4tqk]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4tqk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TQK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4tqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyclocybe_aegerita Cyclocybe aegerita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TQK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tqj|4tqj]], [[4tqm|4tqm]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tqk RCSB], [http://www.ebi.ac.uk/pdbsum/4tqk PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tqk OCA], [https://pdbe.org/4tqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tqk RCSB], [https://www.ebi.ac.uk/pdbsum/4tqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tqk ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/H6CS64_CYCAE H6CS64_CYCAE]
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O-linked N-acetylglucosaminylation (O-GlcNAcylation) is a reversible post-translational modification that plays essential roles in many cellular pathways. Research in this field, however, is hampered by the lack of suitable probes to identify, accumulate, and purify the O-GlcNAcylated proteins. We have previously reported the identification of a lectin from the mushroom Agrocybe aegerita, i.e., Agrocybe aegerita lectin 2, or AAL2, that could bind terminal N-acetylglucosamine with higher affinities and specificity than other currently used probes. In this paper, we report the crystal structures of AAL2 and its complexes with GlcNAc and GlcNAcbeta1-3Galbeta1-4GlcNAc and reveal the structural basis of GlcNAc recognition by AAL2 and residues essential for the binding of terminal N-acetylglucosamine. Study on AAL2 may enable us to design a protein probe that can be used to identify and purify O-GlcNAcylated proteins more efficiently.
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Structural Basis of Specific Recognition of Non-Reducing Terminal N-Acetylglucosamine by an Agrocybe aegerita Lectin.,Ren XM, Li DF, Jiang S, Lan XQ, Hu Y, Sun H, Wang DC PLoS One. 2015 Jun 26;10(6):e0129608. doi: 10.1371/journal.pone.0129608., eCollection 2015. PMID:26114302<ref>PMID:26114302</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Hu, Y L]]
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[[Category: Cyclocybe aegerita]]
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[[Category: Jiang, S]]
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[[Category: Large Structures]]
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[[Category: Lan, X Q]]
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[[Category: Hu YL]]
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[[Category: Li, D F]]
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[[Category: Jiang S]]
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[[Category: Ren, X M]]
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[[Category: Lan XQ]]
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[[Category: Sun, H]]
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[[Category: Li DF]]
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[[Category: Wang, D C]]
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[[Category: Ren XM]]
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[[Category: Complex]]
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[[Category: Sun H]]
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[[Category: Glcnac]]
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[[Category: Wang DC]]
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[[Category: Lectin]]
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[[Category: Sugar binding protein]]
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Current revision

Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection

PDB ID 4tqk

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