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5cv1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5cv1 is ON HOLD Authors: Aoki, S.T., Bingman, C.A., Wickens, M., Kimble, J.E. Description: C. elegans PGL-1 Dimerization Domain [[Category: Unrelea...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==C. elegans PGL-1 Dimerization Domain== | |
| + | <StructureSection load='5cv1' size='340' side='right'caption='[[5cv1]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5cv1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CV1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.599Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cv1 OCA], [https://pdbe.org/5cv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cv1 RCSB], [https://www.ebi.ac.uk/pdbsum/5cv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cv1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PGL1_CAEEL PGL1_CAEEL] Essential role in male and female postembryonic germline development; maternally provided protein maintains a population of proliferating germ cells and zygotic expression is required for correct oogenesis. Predicted to bind RNA.<ref>PMID:9741628</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cellular RNA-protein (RNP) granules are ubiquitous and have fundamental roles in biology and RNA metabolism, but the molecular basis of their structure, assembly, and function is poorly understood. Using nematode "P-granules" as a paradigm, we focus on the PGL granule scaffold protein to gain molecular insights into RNP granule structure and assembly. We first identify a PGL dimerization domain (DD) and determine its crystal structure. PGL-1 DD has a novel 13 alpha-helix fold that creates a positively charged channel as a homodimer. We investigate its capacity to bind RNA and discover unexpectedly that PGL-1 DD is a guanosine-specific, single-stranded endonuclease. Discovery of the PGL homodimer, together with previous results, suggests a model in which the PGL DD dimer forms a fundamental building block for P-granule assembly. Discovery of the PGL RNase activity expands the role of RNP granule assembly proteins to include enzymatic activity in addition to their job as structural scaffolds. | ||
| - | + | PGL germ granule assembly protein is a base-specific, single-stranded RNase.,Aoki ST, Kershner AM, Bingman CA, Wickens M, Kimble J Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201524400. PMID:26787882<ref>PMID:26787882</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5cv1" style="background-color:#fffaf0;"></div> |
| - | [[Category: Bingman | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Caenorhabditis elegans]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Aoki ST]] | ||
| + | [[Category: Bingman CA]] | ||
| + | [[Category: Kimble JE]] | ||
| + | [[Category: Wickens M]] | ||
Current revision
C. elegans PGL-1 Dimerization Domain
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