5cx8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5cx8 is ON HOLD until Paper Publication Authors: Goulas, T., Garcia-Ferrer, I., Hutcherson, J.A., Potempa, B.A., Potempa, J., Scott, D.A., Gomis-Rut...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of RagB, a major immunodominant virulence factor of Porphyromonas gingivalis.== | |
| + | <StructureSection load='5cx8' size='340' side='right'caption='[[5cx8]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5cx8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porphyromonas_gingivalis_W83 Porphyromonas gingivalis W83]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CX8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DO:3-DEOXY-BETA-D-RIBO-HEXOPYRANOSE'>3DO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=RP3:3-DEOXY-D-RIBOFURANOSE-5-PHOSPHATE'>RP3</scene>, <scene name='pdbligand=TG6:6-O-PHOSPHONO-D-TAGATOSE'>TG6</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cx8 OCA], [https://pdbe.org/5cx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cx8 RCSB], [https://www.ebi.ac.uk/pdbsum/5cx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cx8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F5H948_PORGI F5H948_PORGI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Porphyromonas gingivalis is the main causing agent of periodontitis. It deregulates the inflammatory and innate host immune responses through virulence factors, which include the immunodominant outer-membrane surface receptor antigens A (PgRagA) and B (PgRagB), co-transcribed from the rag pathogenicity island. The former is predicted to be a Ton-dependent porin-type translocator but the targets of this translocation and the molecular function of PgRagB are unknown. Phenomenologically, PgRagB has been linked with epithelial cell invasion and virulence according to murine models. It also acts as a Toll-like receptor agonist and promotes multiple mediators of inflammation. Hence, PgRagB is a candidate for the development of a periodontitis vaccine, which would be facilitated by the knowledge of its atomic structure. Here, we crystallized and solved the structure of 54-kDa PgRagB, which revealed a single domain centered on a curved helical scaffold. It consists of four tetratrico peptide repeats (TPR1-4), each arranged as two helices connected by a linker, plus two extra downstream capping helices. The concave surface bears four large intertwined irregular inserts (A-D), which contribute to an overall compact moiety. Overall, PgRagB shows substantial structural similarity with Bacteroides thetaiotaomicron SusD and Tannerella forsythia NanU, which are, respectively, engaged in binding and uptake of maltooligosaccharide/starch and sialic acid. This suggests a similar sugar-binding function for PgRagB for uptake by the cognate PgRagA translocator, and, consistently, three potential monosaccharide-binding sites were tentatively assigned on the molecular surface. This article is protected by copyright. All rights reserved. | ||
| - | + | Structure of RagB, a major immunodominant outer-membrane surface receptor antigen of Porphyromonas gingivalis.,Goulas T, Ferrer IG, Hutcherson JA, Potempa BA, Potempa J, Scott DA, Gomis-Ruth FX Mol Oral Microbiol. 2015 Oct 6. doi: 10.1111/omi.12140. PMID:26441291<ref>PMID:26441291</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Garcia-Ferrer | + | <div class="pdbe-citations 5cx8" style="background-color:#fffaf0;"></div> |
| - | [[Category: Gomis-Ruth | + | == References == |
| - | [[Category: Goulas | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Potempa | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Porphyromonas gingivalis W83]] |
| + | [[Category: Garcia-Ferrer I]] | ||
| + | [[Category: Gomis-Ruth FX]] | ||
| + | [[Category: Goulas T]] | ||
| + | [[Category: Hutcherson JA]] | ||
| + | [[Category: Potempa BA]] | ||
| + | [[Category: Potempa J]] | ||
| + | [[Category: Scott DA]] | ||
Current revision
Structure of RagB, a major immunodominant virulence factor of Porphyromonas gingivalis.
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