5cy0

From Proteopedia

(Difference between revisions)

Current revision

Total Chemical Synthesis, Covalent Structure Verification, and X-ray Structure of Bioactive Ts3 Toxin by Racemic Protein Crystallography

Structural highlights

5cy0 is a 1 chain structure with sequence from Tityus serrulatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.93Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SCX3_TITSE Binds voltage-dependently at site-3 of sodium channels (Nav) and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals (mice).^[1] ^[2] ^[3]

Publication Abstract from PubMed

Ts3 is an alpha scorpion toxin from the venom of the Brazilian scorpion Tityus serrulatus. Ts3 binds to the domain IV voltage sensor of voltage-gated sodium channels (Nav ) and slows down their fast inactivation. The covalent structure of the Ts3 toxin is uncertain, and the structure of the folded protein molecule is unknown. Herein, we report the total chemical synthesis of four candidate Ts3 toxin protein molecules and the results of structure-activity studies that enabled us to establish the covalent structure of biologically active Ts3 toxin. We also report the synthesis of the mirror image form of the Ts3 protein molecule, and the use of racemic protein crystallography to determine the folded (tertiary) structure of biologically active Ts3 toxin by X-ray diffraction.

Elucidation of the Covalent and Tertiary Structures of Biologically Active Ts3 Toxin.,Dang B, Kubota T, Mandal K, Correa AM, Bezanilla F, Kent SB Angew Chem Int Ed Engl. 2016 May 31. doi: 10.1002/anie.201603420. PMID:27244051^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Campos FV, Moreira TH, Beirao PS, Cruz JS. Veratridine modifies the TTX-resistant Na+ channels in rat vagal afferent neurons. Toxicon. 2004 Mar 15;43(4):401-6. PMID:15051403 doi:http://dx.doi.org/10.1016/j.toxicon.2004.01.012
↑ Campos FV, Coronas FI, Beirao PS. Voltage-dependent displacement of the scorpion toxin Ts3 from sodium channels and its implication on the control of inactivation. Br J Pharmacol. 2004 Aug;142(7):1115-22. Epub 2004 Jul 12. PMID:15249424 doi:http://dx.doi.org/10.1038/sj.bjp.0705793
↑ Kirsch GE, Skattebol A, Possani LD, Brown AM. Modification of Na channel gating by an alpha scorpion toxin from Tityus serrulatus. J Gen Physiol. 1989 Jan;93(1):67-83. PMID:2536799
↑ Dang B, Kubota T, Mandal K, Correa AM, Bezanilla F, Kent SB. Elucidation of the Covalent and Tertiary Structures of Biologically Active Ts3 Toxin. Angew Chem Int Ed Engl. 2016 May 31. doi: 10.1002/anie.201603420. PMID:27244051 doi:http://dx.doi.org/10.1002/anie.201603420

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5cy0"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5cy0 is ON HOLD  until Paper Publication
+==Total Chemical Synthesis, Covalent Structure Verification, and X-ray Structure of Bioactive Ts3 Toxin by Racemic Protein Crystallography==
+<StructureSection load='5cy0' size='340' side='right'caption='[[5cy0]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5cy0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tityus_serrulatus Tityus serrulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CY0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CY0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cy0 OCA], [https://pdbe.org/5cy0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cy0 RCSB], [https://www.ebi.ac.uk/pdbsum/5cy0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cy0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SCX3_TITSE SCX3_TITSE] Binds voltage-dependently at site-3 of sodium channels (Nav) and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals (mice).<ref>PMID:15051403</ref> <ref>PMID:15249424</ref> <ref>PMID:2536799</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Ts3 is an alpha scorpion toxin from the venom of the Brazilian scorpion Tityus serrulatus. Ts3 binds to the domain IV voltage sensor of voltage-gated sodium channels (Nav ) and slows down their fast inactivation. The covalent structure of the Ts3 toxin is uncertain, and the structure of the folded protein molecule is unknown. Herein, we report the total chemical synthesis of four candidate Ts3 toxin protein molecules and the results of structure-activity studies that enabled us to establish the covalent structure of biologically active Ts3 toxin. We also report the synthesis of the mirror image form of the Ts3 protein molecule, and the use of racemic protein crystallography to determine the folded (tertiary) structure of biologically active Ts3 toxin by X-ray diffraction.
-Authors: Dang, B., Kubota, T., Mandal, K., Correa, A.M., Bezanilla, F., Kent, S.B.
+Elucidation of the Covalent and Tertiary Structures of Biologically Active Ts3 Toxin.,Dang B, Kubota T, Mandal K, Correa AM, Bezanilla F, Kent SB Angew Chem Int Ed Engl. 2016 May 31. doi: 10.1002/anie.201603420. PMID:27244051<ref>PMID:27244051</ref>
-Description: Total Chemical Synthesis, Covalent Structure Verification, and X-ray Structure of Bioactive Ts3 Toxin by Racemic Protein Crystallography
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Bezanilla, F]]
+<div class="pdbe-citations 5cy0" style="background-color:#fffaf0;"></div>
-[[Category: Kent, S.B]]
+== References ==
-[[Category: Mandal, K]]
+<references/>
-[[Category: Kubota, T]]
+__TOC__
-[[Category: Dang, B]]
+</StructureSection>
-[[Category: Correa, A.M]]
+[[Category: Large Structures]]
+[[Category: Tityus serrulatus]]
+[[Category: Bezanilla F]]
+[[Category: Correa AM]]
+[[Category: Dang B]]
+[[Category: Kent SB]]
+[[Category: Kubota T]]
+[[Category: Mandal K]]

5cy0

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Current revision

Total Chemical Synthesis, Covalent Structure Verification, and X-ray Structure of Bioactive Ts3 Toxin by Racemic Protein Crystallography

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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