5d4k
From Proteopedia
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(New page: '''Unreleased structure''' The entry 5d4k is ON HOLD Authors: Stadtmueller, B.M., Bjorkman, P.J. Description: Crystal structure of the human polymeric Ig Receptor ectodomain [[Category...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the human polymeric Ig receptor (pIgR) ectodomain== | |
| + | <StructureSection load='5d4k' size='340' side='right'caption='[[5d4k]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5d4k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D4K FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.599Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d4k OCA], [https://pdbe.org/5d4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d4k RCSB], [https://www.ebi.ac.uk/pdbsum/5d4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d4k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PIGR_HUMAN PIGR_HUMAN] This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | As a first-line vertebrate immune defense, the polymeric immunoglobulin receptor (pIgR) transports polymeric IgA and IgM across epithelia to mucosal secretions, where the cleaved ectodomain (secretory component; SC) becomes a component of secretory antibodies, or when unliganded, binds and excludes bacteria. Here we report the 2.6A crystal structure of unliganded human SC (hSC) and comparisons with a 1.7A structure of teleost fish SC (tSC), an early pIgR ancestor. The hSC structure comprises five immunoglobulin-like domains (D1-D5) arranged as a triangle, with an interface between ligand-binding domains D1 and D5. Electron paramagnetic resonance measurements confirmed the D1-D5 interface in solution and revealed that it breaks upon ligand binding. Together with binding studies of mutant and chimeric SCs, which revealed domain contributions to secretory antibody formation, these results provide detailed models for SC structure, address pIgR evolution, and demonstrate that SC uses multiple conformations to protect mammals from pathogens. | ||
| - | + | The structure and dynamics of secretory component and its interactions with polymeric immunoglobulins.,Stadtmueller BM, Huey-Tubman KE, Lopez CJ, Yang Z, Hubbell WL, Bjorkman PJ Elife. 2016 Mar 4;5. pii: e10640. doi: 10.7554/eLife.10640. PMID:26943617<ref>PMID:26943617</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Bjorkman | + | <div class="pdbe-citations 5d4k" style="background-color:#fffaf0;"></div> |
| - | [[Category: Stadtmueller | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bjorkman PJ]] | ||
| + | [[Category: Stadtmueller BM]] | ||
Current revision
Crystal structure of the human polymeric Ig receptor (pIgR) ectodomain
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