This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1fhz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:14, 7 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1fhz.gif|left|200px]]
 
-
{{Structure
+
==PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION==
-
|PDB= 1fhz |SIZE=350|CAPTION= <scene name='initialview01'>1fhz</scene>, resolution 2.20&Aring;
+
<StructureSection load='1fhz' size='340' side='right'caption='[[1fhz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=PSO:4&#39;-HYDROXYMETHYL-4,5&#39;,8-TRIMETHYLPSORALEN'>PSO</scene>
+
<table><tr><td colspan='2'>[[1fhz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FHZ FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PSO:4-HYDROXYMETHYL-4,5,8-TRIMETHYLPSORALEN'>PSO</scene></td></tr>
-
}}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhz OCA], [https://pdbe.org/1fhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fhz RCSB], [https://www.ebi.ac.uk/pdbsum/1fhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fhz ProSAT]</span></td></tr>
-
 
+
</table>
-
'''PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION'''
+
__TOC__
-
 
+
</StructureSection>
-
 
+
[[Category: Large Structures]]
-
==Overview==
+
[[Category: Alberti M]]
-
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
+
[[Category: Eichman BF]]
-
 
+
[[Category: Hearst JE]]
-
==About this Structure==
+
[[Category: Ho PS]]
-
1FHZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHZ OCA].
+
[[Category: Mooers BHM]]
-
 
+
-
==Reference==
+
-
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11302703 11302703]
+
-
[[Category: Protein complex]]
+
-
[[Category: Alberti, M.]]
+
-
[[Category: Eichman, B F.]]
+
-
[[Category: Hearst, J E.]]
+
-
[[Category: Ho, P S.]]
+
-
[[Category: Mooers, B H.M.]]
+
-
[[Category: PSO]]
+
-
[[Category: cross-linked dna]]
+
-
[[Category: dna-drug complex]]
+
-
[[Category: holliday junction]]
+
-
[[Category: psoralen]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 11:47:24 2008''
+

Current revision

PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION

PDB ID 1fhz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fhz"
Personal tools