This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3j9k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:41, 7 February 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Structure of Dark apoptosome in complex with Dronc CARD domain==
==Structure of Dark apoptosome in complex with Dronc CARD domain==
-
<StructureSection load='3j9k' size='340' side='right' caption='[[3j9k]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
+
<SX load='3j9k' size='340' side='right' viewer='molstar' caption='[[3j9k]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9K FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9K FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
-
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j9l|3j9l]]</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [https://pdbe.org/3j9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [https://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum]</span></td></tr>
+
</table>
</table>
-
{{Large structure}}
 
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>
+
[https://www.uniprot.org/uniprot/Q7KLI1_DROME Q7KLI1_DROME]
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Apoptosis is executed by a cascade of caspase activation. The autocatalytic activation of an initiator caspase, exemplified by caspase-9 in mammals or its ortholog, Dronc, in fruit flies, is facilitated by a multimeric adaptor complex known as the apoptosome. The underlying mechanism by which caspase-9 or Dronc is activated by the apoptosome remains unknown. Here we report the electron cryomicroscopic (cryo-EM) structure of the intact apoptosome from Drosophila melanogaster at 4.0 A resolution. Analysis of the Drosophila apoptosome, which comprises 16 molecules of the Dark protein (Apaf-1 ortholog), reveals molecular determinants that support the assembly of the 2.5-MDa complex. In the absence of dATP or ATP, Dronc zymogen potently induces formation of the Dark apoptosome, within which Dronc is efficiently activated. At 4.1 A resolution, the cryo-EM structure of the Dark apoptosome bound to the caspase recruitment domain (CARD) of Dronc (Dronc-CARD) reveals two stacked rings of Dronc-CARD that are sandwiched between two octameric rings of the Dark protein. The specific interactions between Dronc-CARD and both the CARD and the WD40 repeats of a nearby Dark protomer are indispensable for Dronc activation. These findings reveal important mechanistic insights into the activation of initiator caspase by the apoptosome.
+
-
 
+
-
Structure of the apoptosome: mechanistic insights into activation of an initiator caspase from Drosophila.,Pang Y, Bai XC, Yan C, Hao Q, Chen Z, Wang JW, Scheres SH, Shi Y Genes Dev. 2015 Feb 1;29(3):277-87. doi: 10.1101/gad.255877.114. PMID:25644603<ref>PMID:25644603</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
-
</StructureSection>
+
</SX>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
-
[[Category: Bai, X]]
+
[[Category: Large Structures]]
-
[[Category: Chen, Z]]
+
[[Category: Bai X]]
-
[[Category: Hao, Q]]
+
[[Category: Chen Z]]
-
[[Category: Pang, Y]]
+
[[Category: Hao Q]]
-
[[Category: Scheres, S H.W]]
+
[[Category: Pang Y]]
-
[[Category: Shi, Y]]
+
[[Category: Scheres SHW]]
-
[[Category: Wang, J]]
+
[[Category: Shi Y]]
-
[[Category: Yan, C]]
+
[[Category: Wang J]]
-
[[Category: Apoptosis]]
+
[[Category: Yan C]]
-
[[Category: Caspase activation]]
+
-
[[Category: Programmed cell death]]
+

Current revision

Structure of Dark apoptosome in complex with Dronc CARD domain

3j9k, resolution 4.10Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j9k"
Personal tools