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3j79

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==Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine, large subunit==
==Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine, large subunit==
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<StructureSection load='3j79' size='340' side='right' caption='[[3j79]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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<SX load='3j79' size='340' side='right' viewer='molstar' caption='[[3j79]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j79]] is a 44 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. This structure supersedes and combines the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx6 1vx6] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx7 1vx7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J79 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J79 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j79]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx6 1vx6] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vx7 1vx7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J79 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j7a|3j7a]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j79 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j79 RCSB], [http://www.ebi.ac.uk/pdbsum/3j79 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j79 OCA], [https://pdbe.org/3j79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j79 RCSB], [https://www.ebi.ac.uk/pdbsum/3j79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j79 ProSAT]</span></td></tr>
</table>
</table>
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{{Large structure}}
 
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/C0H4L5_PLAF7 C0H4L5_PLAF7]] Binds to the 23S rRNA (By similarity).[RuleBase:RU000576]
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[https://www.uniprot.org/uniprot/Q8I3T9_PLAF7 Q8I3T9_PLAF7]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Malaria inflicts an enormous burden on global human health. The emergence of parasite resistance to front-line drugs has prompted a renewed focus on the repositioning of clinically approved drugs as potential anti-malarial therapies. Antibiotics that inhibit protein translation are promising candidates for repositioning. We have solved the cryo-EM structure of the cytoplasmic ribosome from the human malaria parasite, Plasmodium falciparum, in complex with emetine at 3.2 A resolution. Emetine is an anti-protozoan drug used in the treatment of ameobiasis that also displays potent anti-malarial activity. Emetine interacts with the E-site of the ribosomal small subunit and shares a similar binding site with the antibiotic pactamycin, thereby delivering its therapeutic effect by blocking mRNA/tRNA translocation. As the first cryo-EM structure that visualizes an antibiotic bound to any ribosome at atomic resolution, this establishes cryo-EM as a powerful tool for screening and guiding the design of drugs that target parasite translation machinery.
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Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine.,Wong W, Bai XC, Brown A, Fernandez IS, Hanssen E, Condron M, Tan YH, Baum J, Scheres SH Elife. 2014 Jun 9:e03080. doi: 10.7554/eLife.03080. PMID:24913268<ref>PMID:24913268</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
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</div>
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Plasmodium falciparum]]
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[[Category: Large Structures]]
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[[Category: Bai, X C]]
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Baum, J]]
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[[Category: Bai XC]]
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[[Category: Brown, A]]
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[[Category: Baum J]]
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[[Category: Condron, M]]
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[[Category: Brown A]]
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[[Category: Fernandez, I S]]
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[[Category: Condron M]]
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[[Category: Hanssen, E]]
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[[Category: Fernandez IS]]
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[[Category: Scheres, S H.W]]
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[[Category: Hanssen E]]
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[[Category: Tan, Y H]]
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[[Category: Scheres SHW]]
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[[Category: Wong, W]]
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[[Category: Tan YH]]
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[[Category: Emetine]]
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[[Category: Wong W]]
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[[Category: Ribosome-inhibitor complex]]
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Current revision

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine, large subunit

3j79, resolution 3.20Å

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